国際学会

• Globalization of Pharmaceutics Education Network 2022 (GPEN2022), (Minneapolis),October 19-22 (2022)

• Investigation of in vivo behavior of extracellular vesicles with different sizes after intraduodenal administration
  Yuki Kobayashi, Momoko Naiki, Ayane Yabuta Yuki Takahashi, Yoshinobu Takakura.
  

• The 48th International Symposium on Nucleic Acids Chemistry (ISNAC2021), (Online),November 10-12 (2021)
  • Immunostimulatory Activity of DNA Dendrimers
    Yusuke Kawamoto, Wen Liu, Ji Hye Yum, Soyoung Park, Hiroshi Sugiyama, Yuki Takahashi, Yoshinobu Takakura.
• 2021 CSPS/PSJ/CC-CRS SYMPOSIUM, (Online), June 1-4 (2021)
  • Development of DNA supramolecules utilizing rolling circle amplification and hydrophobic interaction for efficient immunotherapy
    Koichi Ito, Mutsumi Kariya, Yuki Takahashi, Yoshinobu Takakura.
  • Development of allergic rhinitis immunotherapy using antigen-loaded multifunctional small extracellular vesicles
    Wen Liu, Maki Ota, Yuki Takahashi, Yoshinobu Takakura.
  • Development of a small extracellular vesicle (sEV) labeling method utilizing fusion protein of fluorescent proteins and sEV-tropic protein for the detection of phosphatidylserine status of sEVs
    Yuki Kobayashi, Yuki Takahashi, Yoshinobu Takakura.

• International Society for Extracellular Vesicles 2019 (ISEV2019), (Kyoto, Japan), April 24-28 (2019)
  • Development of CD40L-modified small extracellular vesicles for the effective induction of anti-tumor immune response.
    Wen L, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.
  • Development of Interferon γ-loaded tumor cell-derived extracellular vesicles applicable to cancer vaccine therapy.
    Nakagawa N, Takahashi Y, Takakura Y.

• Globalization of Pharmaceutics Education Network 2018 (GPEN2018), (Singapore), September 26-29 (2018)
  • Development of Exosome Assembly Formed by Utilization of DNA Hybridization and its Application for Cancer Vaccine.
    Matsumoto A, Takahashi Y, Ariizumi R, Nishikawa M, Takakura Y.
  • Development of A Nanostructured RNA/DNA Adjuvant Targeting Toll-like Receptor 7/8.
    Komura F, Nishikawa M, Takahashi Y, Takakura Y.

• The 10th Seoul-Kyoto-Osaka Joint Symposium On Pharmaceutical Sciences For Young Scientists, (Osaka, Japan), May 22-24 (2017)
  • Development of exosome-based nucleic acid delivery systems.
    Yamashita T, Takahashi Y, Nishikawa M, Takakura Y.
    
• 6th Annual Meeting of the International Society for Extracellular Vesicles (ISEV), (Toronto, Canada), May 18-21 (2017)
  • Yield, physicochemical properties, and pharmacokinetics of exosomes derived from mouse cell lines.
    Charoenviriyakul C, Takahashi Y, Morishita M, Matsumoto A, Nishikawa M, Takakura Y.
    
  • Melanoma progression effects on biodistribution of B16BL6-derived exosomes from tumors and biological role of B16BL6-derived exosomes.
    Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.
    
• International Syposium on Drug Delivery and Pharmaceutical Sciences: Beyond the History (ISDDPS), (Kyoto, Japan), March 9-10 (2017)
  • Recent Advances in Nucleic acid Drug Delivery
    Yoshinobu Takakura

• Globalization of Pharmaceutics Education Network 2016 (GPEN2016), (Lawrence, KS, USA), November 9-12 (2016)
  • Sustained expression system of interferon-beta by using interferon-inducible Mx promoter for interferon-beta gene therapy.
    Atsushi Hamana, Yuki Takahashi, Makiya Nishikawa, Yoshinobu Takakura
    (Short course: Challenges of Stabilizing Therapeutic Proteins)
  • Elucidation of factors determining the cell distribution in artificially fabricated mixed multicellular spheroids for improved insulin secretion.
    Yuya Mizukami, Makiya Nishikawa, Yuki Takahashi, Kazunori Shimizu, Satoshi Konishi, Yoshinobu Takakura
• The 1st Workshop for Japan-Korea Young Scientists on Pharmaceutics, (Kyoto, Japan), June 24-25 (2016)
  • Role of phosphatidylserine-derived negative surface charge in the recognition of B16BL6-derived exosomes by macrophages.
    Akihiro Matsumoto, Yuki Takahashi, Makiya Nishikawa, Kohei Sano, Masaki Morishita, Chonlada Charoenviriyakul, Hideo Saji, Yoshinobu Takakura.
    Poster Presentation Award受賞
  • Enhanced Activity of Immunosuppressive Oligodeoxynucleotides by Incorporating into Hexapod-like Nanostructured DNA.
    Yuki Araie, Yuki Takahashi, Yoshinobu Takakura, Makiya Nishikawa
    
• 5th Annual Meeting of the International Society for Extracellular Vesicles (ISEV), (Rotterdam, the Netherlands), May 4-7 (2016)
  • Induction of potent antitumor immune response by development of CpG DNA-conjugated tumor cell-derived exosomes.
    Morishita M, Takahashi Y, Nishikawa M, Takakura Y.
  • Effect of exosome isolation methods on their yield, physicochemical and pharmacokinetic properties.
    Yamashita T, Takahashi Y, Nishikawa M, Takakura Y.
• University Bordeaux-Kyoto University Mini-Symposium on Biomolecular Science, iCeMS, Kyoto University, (Kyoto, Japan), January 25 (2016)
  • Polypod-like structured DNA for delivery of bioactive compounds.
    Nishikawa M.

• The Fourth International Meeting of The International Society for Extracellular Vesicles 2015 (ISEV2015), (Washington, USA), April 23-26 (2015)
  • Identification and screening of exosome-tropic RNA by Selex method
    and its application in loading therapeutic RNA to exosomes.
    Takahashi Y, Shinotsuka H, Kato K, Nishikawa M, Takakura Y.
• The 5th International Symposium on Carcinogenic Spiral Infection, Immunity, and Cancer, (Kobe, Japan), February 26-27 (2015)
  • Self-gelatinizable Nucleic Acid-based Sustained Delivery of Immunostimulatory DNA and Antigen for Induction of Antitumor Immunity in Mice.
    Umeki Y, Nishikawa M.

• 10th Annual Meeting of the Oligonucleotide Therapeutics Society, (San Diego, USA), October 12-15 (2014)
  • DNA Hydrogel-based Sustained Delivery of Immunostimulatory DNA and Antigen for Induction of Antitumor Immunity in Mice.
    Umeki Y, Nishikawa M, Mohri K, Takahashi Y, Takakura Y.

• Globalization of Pharmaceutics Education Network 2014 (GPEN2014), (Helsinki, Finland), August 27-30 (2014)
  • Quantitative analysis of invivo fate of mouce melanoma B16BL6-derived exosomes after intravenous injection into mice by development of labeling method for exosomes.
    Morishita M, Takahashi Y, Imai T, Nishikawa M, Takakura Y.
  • Optimization of DNA nanostructure for delivery of nucleic acid drugs to immune cells.
    Ohtsuki S, Nishikawa M, Matsuzaki N, Mohri K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y.
    (Short course: Nucleic acid and gene delivery section)

• The 8th Seoul-Kyoto-Osaka Joint Symposium On Pharmaceutical Sciences For Young Scientists, (Osaka), June 10 (2014)
  • Development of a quantitative and sensitive method to label exosomes for analysis of their in vivo fate.
    Morishita M, Takahashi Y, Nishikawa M, Sano K, Saji H, Takakura Y.

• International Society for Extracellular Vesicles 2014, (Rotterdam), April 30-May 3 (2014)
  • Quantitative analysis of the distribution and clearance of mouse melanoma B16-BL6-derived exosomes in mice.
    Takahashi Y, Imai T, Nishikawa M, Kato K, Morishita M, Takakura Y.

• 5th FIP Pharmaceutical Sciences World Congress, (Melbourne, Australia), April 13-16 (2014)
  • Quantitative evaluation of in vivo fate of mouse melanoma B16-BL6-derived exosomes after intravenous injection into mice.
    Takahashi Y, Imai T, Nishikawa M, Kato K, Morishita M, Takakura Y.

2013 年

• 9th Annual Meeting of the Oligonucleotide Therapeutics Society, (Naples, Italy), October 6-8 (2013)

  • Development of Self-Assembling and Immunostimulatory Dendrimer-like DNA for Stimulation of Immune Cells.
    Nishikawa M, Kusuki E, Mohri K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y

2012 年

• Globalization of Pharmaceutics Education Network 2012, (Melborne, Australia), November 28-December 1 (2012)

  • Complete elimination of hepatitis C virus by sustained nonviral gene delivery of human interferon γ in human hepatocyte chimeric mice.
    M. Ando, Y. Takahashi, M. Nishikawa, N. Hiraga, M. Imamura,
    K. Chayama, Y. Takakura

• 8th Annual Meeting of the Oligonucleotide Therapeutics Society (Boston, USA), Oct 28-31 (2012)

  • Visualization of Polypod-like Structured DNA, Polypodna, and Its Application as Nano-sized Immunomodulator and Building Block for Versatile DNA Hydrogel.
    Makiya Nishikawa, Masayuki Endo, Hiroshi Sugiyama,
    Yuki Takahashi, Yoshinobu Takakura

• American Society of Gene & Cell Therapy 15th Annual Meeting and Course (Philadelphia, PA), May 16-19 (2012)

  • Long-term elimination of human hepatitis C virus in human hepatocyte chimeric mice after single administration of plasmid DNA expressing human interferon-γ.
    Yuki Takahashi, Mitsuru Ando, Makiya Nishikawa, Nobuhiko Hiraga, Michio Imamura, Kazuaki Chayama and Yoshinobu Takakura
    

2011 年

• 7th Annual Meeting of the Oligonucleotide Therapeutics Society (Copenhagen, Denmark), September 8-10 (2011)

  • Kinetic comparison of gene silencing profile of siRNA and shRNA-expressing plasmid DNA in vivo.
    Yuki Takahashi, Yuriko Matsui, Makiya Nishikawa, Yoshinobu Takakura.
    
  • Development of DNA nanogels and hydrogels as injectable delivery systems for CpG DNA and other nucleic acid-based drugs.
    Makiya Nishikawa, Kohta Mohri, Kohei Ogawa, Natsuki Takahashi, Yuki Takahashi, Yoshinobu Takakura

  　
• 38th Annual Meeting and Exposition of the Controlled Release Society (National Harbor, Maryland USA), July 30 - August 3 (2011)

  • Optimization of interferon-γ cancer gene therapy by regulating transgene expression profile or controlling the tissue distribution of transgene products.
    Yoshinobu Takakura (Invited Speaker)

• The 6th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists (Seoul, Korea), June 2-4 (2011)

  • Enhancement of therapeutic effect of cell-based therapy by increasing survival of transplanted cells using synthetic cell adhesion molecules.
    Kousuke Kusamori, Makiya Nishikawa, Tetsuya Suehara, Yuki Takahashi, Sayumi Yamazoe, Motonari Uesugi, Yoshinobu Takakura

  　

• American Society of Gene & Cell Therapy 14^th Annual Meeting (Seattle WA, USA), May 18-21 (2011)

  • Design and hydrodynamic gene transfer of ‘sticky’ IFNγ for liver-directed gene therapy.
    Mitsuru Ando, Yuki Takahashi, Hanae Mukumoto, Makiya Nishikawa, Yoshihiko Watanabe and Yoshinobu Takakura.
    

2010 年

• Pharmaceutical Sciences World Congress 2010 (New Orleans, USA), November 14-18 (2010)

  • Interferon γ-mediated inhibition of nuclear entry of plasmid vector and mRNA transcription from the vector: an obstacle for repeated injections of interferon γ-expressing plasmid vector
    Lei Zang, Makiya Nishikawa, Kazuya Machida, Mitsuru Ando, Yuki Takahashi, Yoshinobu Takakura.
    
  • Design and gene delivery of mouse interferon γ genetically fused with mouse serum albumin
    Noriko Miyakawa, Makiya Nishikawa, Yuki Takahashi, Yoshihiko Watanabe, Yoshinobu Takakura
    
  • Design and gene delivery of ‘sticky’ interferonγ to increase the effect/side-effect ratio for liver-directed interferon-γ gene therapy
    Mitsuru Ando, Yuki Takahashi, Hanae Mukumoto, Makiya Nishikawa, Yoshihiko Watanabe, Yoshinobu Takakura

  　　

• Globalization of Pharmaceutics Education Network: Eighth Meeting, 2010 (GPEN2010), 　　　　　(North　Carolina,USA) November 10-12 (2010)

  • Structural and immunological properties of polypod-like structure forming nucleic acids
    Kohta Mohri, Makiya Nishikawa, Nao Matsuoka, Natsuki Takahashi, Yuki Takahashi, Yoshinobu Takakura.
    
  • Acceleration of wound healing by increasing survival of transplanted cells using adhsamine, a synthetic cell adhesion molecule
    Kousuke Kusamori, Makiya Nishikawa, Tetsuya Suehara, Yuki Takahashi, Sayumi Yamazoe, Motonari Uesugi, Yoshinobu Takakura

  　
• 6^th Annual Meeting of Oligonucleotide Therapeutics Society (Dana Point, USA) October 20-23 (2010)

  • Pod number-dependent increase in the immunostimulatory activity of CpG DNA formulated in polypod-like structures
    Makiya Nishikawa, Kohta Mohri, Nao Matsuoka, Kouhei Ogawa, Yuki Takahashi and Yoshinobu Takakura

  　
• American Society of Gene & Cell Therapy 13^th Annual Meeting (Washington DC, USA), May 19-22 (2010)

  • Increased therapeutic effect of cell transplantation for wound healing in diabetic mice by prolonging the survival of transplanted cells using adhesamine, a synthetic adhesion molecule
    Tetsuya Suehara, Makiya Nishikawa, Yuki Takahashi, Sayumi Yamazoe, Motonari Uesugi, Yoshinobu Takakura.
    
  • Prevention of atopic dermatitis by plasmid-based sustained transgene expression of interferon γ in NC/Nga mice
    Kayoko Hattori, Makiya Nishikawa, Kanitta Watcharanurak, Akihiko Ikoma, Kenji Kabashima, Hiroyasu Toyota, Yuki Takahashi, Rei Takahashi, Yoshihiko Watanabe, Yoshinobu Takakura

2009 年

• 36th Annual Meeting and Exposition of the Controlled Release Society (Copenhagen, Denmark), July 18-22 (2009)

  • Design and development of DNA-based nanoassemblies and hydrogels with enhanced immunostimulatory activity
    Sakulrat Rattanakiat, Makiya Nishikawa, Yumiko Mizuno, Mitsuhiro Matono, Hisakage Funabashi, Dan Luo and Yoshinobu Takakura

2008 年

• Ehrlich II - 2nd World Conference on Magic Bullets, N?rnberg, (Germany), October 3-5 (2008)

  • Interferon Gene Therapy against Metastatic Cancer
    Yoshinobu Takakura, Makiya Nishikawa(invited)

• 2008 KCRS Annual Conference: Research Networking for Future Therapy, Jeju Island (Korea), September 4-5 (2008)

  • Optimization of Design and Delivery of Plasmid Vector for Interferon Gene Therapy
    Yoshinobu Takakura(invited)

• American Society of Gene Therapy 11th Annual Meeting, Seattle (Boston, MA), May 28- June 1 (2008)

  • Reactivation of silenced transgene expression in mouse by rapid, large-volume injection of isotonic solution
    Makiya Nishikawa, Yuki Takahashi, Ayumi Nakayama, Yasushi Fukuhara, Yoshinobu Takakura

• 2nd Asian Pacific Regional Meeting of International Society of Xenobiotics (Shanghai, China), May 11-13 (2008)

  • Receptor-mediated delivery of drugs, proteins and genes (tentative)
    Makiya Nishikawa (Invited)

• The 4th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists, (Kyoto), April 22-24 (2008)

  • Unique property of IFN- production upon intravenous injection of CpG plasmid DNA/cationic liposome complex
    Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda, Yumiko Mizuno, Hiroyasu Toyota and Yoshinobu Takakura

• Pan-Pacific International Partnership Conference on Pharmaceutical and Life Sciences (The 4th US-Japan Joint Conference) (Nagoya), Febrary 22-23 (2008)

  • Nonviral gene delivery based on physical methods in combination with naked plasmid DNA
    Yoshinobu Takakura (Invited).
    
  • Comparison of skin and spleen as injection site of vaccines for induction of immune response
    Xin Guan, Makiya Nishikawa, Yoshinobu Takakura
    
  • Increasing anticancer activity of interferon gene transfer by prolonging the duration of transgene expression
    Lei Zang, Makiya Nishikawa, Masaru Mitsui, Yuki Takahashi, Yoshinobu Takakura

2007 年

• American Society of Gene Therapy 10th Annual Meeting, Seattle (USA), May 30- June 3 (2007)

  • Inhibition of hepatic metastasis of colon carcinoma cells by RNA interference-mediated suppression of HIF-1α in tumor cells and hepatocytes
    Yuki Takahashi, Makiya Nishikawa and Yoshinobu Takakura
    
  • Deletion of CpG motifs from plasmid DNA for sustained transgene expression in vivo: evaluation based on a statistical analysis of the expression profile
    Makiya Nishikawa, Seiji Takemoto, Kiyoshi Yamaoka, Masaru Mitsui, Hisoon Lee and Yoshinobu Takakura
    
  • Heat shock protein 70-antigen fusion protein expressing DNA vaccin for cancer immunotherapy
    Ayumi Yamaoka, Seiji Takemoto, Makiya Nishikawa, Tomoya Yata, yuji Ohno and Yoshinobu Takakura

• Pharmaceutical Sciences World Congress, Amsterdam (The Netherlands), April 22-25 (2007)

  • Silencing of SOCS genes in cancer cells for effective interferon cancer therapy
    Yuki Takahashi, Makiya Nishikawa, Haruka Kaneda, Nana Takasuka, Kayoko Hattori and Yoshinobu Takakura

• Young Pharmaceutical Scientists Meet in Amsterdam, Amsterdam (The Netherlands), April 20-21 (2007)

  • Inhibition of hepatic metastasis of colon carcinoma cells by RNA interference-mediated suppression of HIF-1α in tumor cells and hepatocytes
    Yuki Takahashi, Makiya Nishikawa and Yoshinobu Takakura
    
  • Conribution of macrophage-like cells to cytokine secretion after intravenous injection of naked or complexed plasmid DNA
    Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda, Yumiko Mizuno and Yoshinobu Takakura
    
  • Formulation-dependent characteristics of cytokine secretion in primary cultured cells and in mice stimulated with plasmid DNA
    Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda, Yumiko Mizuno and Yoshinobu Takakura

2006 年

• Globalization of Pharmaceutics Education Network 2006, Kansas (USA), October 25-27 (2006)

  • Enhanced CTL generation by HSP70 fusion protein harboring both MHC class I and class II antigenic peptides
    Seiji Takemoto, Makiya Nishikawa, Atsushi Ota and Yoshinobu Takakura
    
  • Cytokine secretion profiles in primary cultured cells and in mice stimulated with plasmid DNA
    Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda and Yoshinobu Takakura

• The 2nd UK-Japan Gene Therapy Workshop 2006, Tokyo (Japan), October 12 (2006)

  • Efficient delivery of therapeutic genes and small interfering RNA by intravascular injection of naked plasmid DNA
    Makiya Nishikawa (Invited)

• Mini-Symposium for Nano-Bio Drug Delivery System, Seoul(Korea), September 1 (2006)

  • Design and Delivery of Plasmid DNA for In Vivo Cancer Gene Therapy and Genetic Vaccination
    Yoshinobu Takakura (Invited)

• The First FIP-APSTJ Joint Workshop on Gene Delivery, Sapporo (Japan), July 10-12 (2006)

  • In vivo plasmid DNA delivery for cancer genetherapy and genetic vaccination
    Yoshinobu Takakura (Invited)
    
  • Development of Hsp70-based DNA vaccines for cancer immunotherapy
    Seiji Takemoto, Makiya Nishikawa, Takayuki Otsuki, Ayumi Yamaoka, Atsushi Ota and Yoshinobu Takakura

• The 3rd Korea-Japan Joint Symposium on Drug Delivery and Therapy, Seoul (Korea), April 20-21 (2006)

  • Inhibition of tumor metastasis by suppressing the expression of proteins associated with metastastic tumor growth
    Makiya Nishikawa (Invited)

• American Society of Gene Therapy 9th Annual Meeting, Baltimore (USA), May 31- June 4 (2006)

  • Induction of antitumor immunity by cell-penetrating peptide-coupled Hsp70 gene transfer to tumor cells.
    Takayuki Otsuki, Makiya Nishikawa, Atsushi Ota, Seiji Takemoto and Yoshinobu Takakura
    
  • Increased transgene expression of dystrophine in mdx muscle by RNAi-mediated silencing of calpain expression
    Makiya Nishikawa, Kazuhiro Hirata, Kazuya Machida, Yuki Takahashi, Katsutoshi Yuasa, Shinichi Takeda and Yoshinobu Takakura
    
  • Enhancement of antitumor activity of interferons by RNAi-mediated silencing of suppressors of cytokines signaling in tumor cells.
    Yuki Takahashi, Haruka Kaneda, Makiya Nishikawa and Yoshinobu Takakura

2005 年

• 2nd Seoul-Osaka-Kyoto Joint Symposium on Pharmaceutical Sciences for young Scientists 2005, Osaka (Japan), December 4-6 (2005)

  • Generation of cytotoxic T lymphocyte by delivery of MHC class I epitope to antigen presenting cells using polyhistidine-coupled mouse heat shock protein 70.
    Seiji Takemoto, Makiya Nishikawa, Takayuki Otsuki, Ayumi Yamaoka, Kazuya Maeda, Atsushi Ota and Yoshinobu Takakura

• 1st Indo-Japanese International Conference on Advances in Pharmaceutical Research and Technology, Mumbai (India), November 25-29 (2005)

  • Plasmid DNA delivery for in vivo cancer gene therapy.
    Yoshinobu Takakura (Invited)

• 30th FEBS Congress & 9th IUBMB Conference, Budapest, (Hungary), July 2-7 (2005)

  • Chemically modified catalase for prevention of ROS-mediated injury and tumor metastasis
    Makiya Nishikawa

• 8th Annual Meeting of American Society of Gene Therapy, St. Louis, (USA), June 1-5 (2005)

  • Enhanced anti-tumor effect of interferon gene transfer by sustained transgene expression using CpG-reduced plasmid DNA
    Hiroki Kawano, Makiya Nishikawa, Masaru Mitsui and Yoshinobu Takakura
  • Intratumoral delivery of small interfering RNA for inhibition of tumor progression in mice
    Yuki Takahashi, Makiya Nishikawa and Yoshinobu Takakura

• 12th International Symposium of Recent Advances in Drug Delivery Systems, Salt Lake City (USA), Feb 21 - 24 (2005)

  • Inhibition of metastatic tumor growth by targeted delivery of antioxidant enzymes
    Makiya Nishikawa, Yoshinobu Takakura, Mitsuru Hashida
    
  • Biological responses to immunostimulatory DNA and its application for inhibition of peritoneal dissemination of tumor cells
    Makiya Nishikawa, Yukari Umeyama, Keiko Kako, Mitsuru Hashida and Yoshinobu Takakura
    
  • Development of heat shock protein 70-based tumor vaccine: pharmacokinetic considerations and antigen delivery.
    Seiji Takemoto, Takayuki Otsuki, Ayumi Yamaoka, Makiya Nishikawa and Yoshinobu Takakura
    

2004 年

• 2nd World Congress of the Board of Pharaceutical Sciences of FIP (PSWC2004), Kyoto (Japan) 30 May - 3 June (2004)

  • Increased dendritic cell migration to the vaccination site and enhanced immune response by polyplex-based DNA vaccine
    Atsushi Kawase, Ayumi Yamaoka, Makiya Nishikawa and Yoshinobu Takakura
    
  • Involvement of scavenger receptor and clathrin-mediated endocytosis in plasmid DNA uptake by mouse macrophages and dendritic cells
    Takaharu Yoshinaga, Haruka Kaneda, Yoshiyuki Ogawa, Makiya Nishikawa and Yoshinobu Takakura
    
  • Reduced DNA uptake and degradation in antigen-presenting cells from systemic lupus erythematosus model mice
    Yoshiyuki Ogawa, Takaharu Yoshinaga, Makiya Nishikawa and Yoshinobu Takakura
    
  • Induction of cytotoxic T lymphocytes following immunization with cationized soluble antigen
    Tomoko Ikenaga, Makiya Nishikawa and Yoshinobu Takakura
    
  • Control of intracellular trafficking of genetically delivered antigens in dendritic cells for improved DNA vaccination
    Keiko Isaji, Makiya Nishikawa, Atsushi Kawase, Ayumi Yamaoka and Yoshinobu Takakura
    
  • Acute inflammatory response to nonviral vectors in mice is mediated by antigen presenting cells
    Keiko Kako, Makiya Nishikawa and Yoshinobu Takakura
    
  • Therapeutic effects of interferon gene delivery by CpG-reduced plasmid DNA
    Hiroki Kawano, Naoki Kobayashi, Makiya Nishikawa and Yoshinobu Takakura
    
  • Gene silencing in primary and metastatic tumors by siRNA-expressing vector
    Yuki Takahashi, Makiya Nishikawa, Naoki Kobayashi, Makoto Miyagishi*, Kazunari Taira* and Yoshinobu Takakura (*Graduate School of Engin. Univ. of Tokyo.)
    
  • Uptake of plasmid DNA and subsequent cellular activation in human macrophages
    Yuga Fukuhara, Ikuko Yamane, Takaharu Yoshinaga, Makiya Nishikawa and Yoshinobu Takakura
    
  • Tissue distribution characteristics of mouse heat shock protein 70, a possible immune activator for tumor therapy
    Takayuki Otsuki, Seiji Takemoto, Makiya Nishikawa and Yoshinobu Takakura
    
  • Effects of cholesterol depletion on secretion polarity of human interferon- b in Mardin-Darby canine kidney cells
    Kayo Ishida, Masato Maruyama, Yoshihiko Watanabe and Yoshinobu Takakura
    
  • Hydrodynamics-based efficient intracellular gene delivery involves a nonspecific process via the hepatic cellular membrane of transient hyperpermeability
    Kazuhiro Hirata, Naoki Kobayashi, Makiya Nishikawa and Yoshinobu Takakura
    
  • Vector-based in vivo RNA interference targeting exogenous and endogenous genes
    Hiroyuki Yoshida, Naoki Kobayashi, Yumi Matsui, Kazuhiro Hirata, Makoto Miyagishi*, Kazunari Taira*, Makiya Nishikawa and Yoshinobu Takakura (*Graduate School of Engin. Univ. of Tokyo.)
    

• 5th Globalization of Pharmaceutics Education Network (GPEN) International Graduates Student Meeting, Kyoto (Japan), May 26-28 (2004)

  • In vivo manipulation of dendritic cell migration and activation to elicit immune response by polyplex-based DNA vaccination
    Atsushi Kawase, Ayumi Yamaoka, Makiya Nishikawa and Yoshinobu Takakura
    
  • Mechanism of plasmid DNA uptake by mouse macrophages and dendritic cells
    Takaharu Yoshinaga, Haruka Kaneda, Yoshiyuki Ogawa, Makiya Nishikawa and Yoshinobu Takakura
    
  • Pathognomonic DNA Processing in Antigen-Presenting Cells from Lupus-Prone NZB x NZW F ₁ Mice
    Yoshiyuki Ogawa, Takaharu Yoshinaga, Makiya Nishikawa and Yoshinobu Takakura
    
  • Tissue distribution characteristics of mouse heat shock protein 70; CD91 is the main uptake receptor in the liver
    Seiji Takemoto, Takayuki Otsuki, Makiya Nishikawa and Yoshinobu Takakura
    
  • Improved antigen presentation efficiency by dendritic cells with plasmid DNA encoding structure-modified antigens
    Keiko Isaji, Makiya Nishikawa, Atsushi Kawase, Ayumi Yamaoka and yoshinobu Takakura
    
  • Contribution of macrophages to the acute inflammatory responses following nonviral vector administration
    Keiko Kako, Makiya Nishikawa and Yoshinobu Takakura
    
  • Antitumor activities of interferon gene delivery by CpG-reduced plasmid DNA in murine metastasis models
    Hiroki Kawano, Makiya Nishikawa and Yoshinobu Takakura
    
  • Delivery of siRNA and siRNA-expressing vector to primary and metastatic tumors for induction of in vivo RNA interference
    Yuki Takahashi, Makiya Nishikawa, Naoki Kobayashi, Makoto Miyagishi, Kazunari Taira and Yoshinobu Takakura
    
  • CpG-motif independent activation of human macrophages derived from peripheral blood mononuclear cells by DNA/cationic liposome
    Yuga Fukuhara, Ikuko Yamane, Takaharu Yoshinaga, Makiya Nishikawa and Yoshinobu Takakura
    
  • Mechanism of Polarized Secretion Erythropoietin in Mardin-Darby Canine Kidney Cells
    Kayo Ishida, Masato Maruyama, Yoshihiko Watanabe and Yoshinobu Takakura
    
  • Mechanism of Plasmid DNA Delivery by Hydrodynamics-Based Procedure
    Kazuhiro Hirata, Naoki Kobayashi, Makiya Nishikawa and Yoshinobu Takakura
    
  • Sequence-specific suppression of the expression of exogenous and endogenous genes in mice via RNA interference
    Hiroyuki Yoshida, Naoki Kobayashi, Yumi Matsui, Kazuhiro Hirata, Makoto Miyagishi, Kazanari Taira, Makiya Nishikawa and Yoshinobu Takakura
    

2002 年

• 4th Globalization of Pharmaceutics Education Network (GPEN) International Graduates Student Meeting, Michigan (USA), November 6-8 (2002)

  • Plasmid DNA Uptake and Subsequent Induction of Inflammatory Cytokines in Cultured Mouse Macrophages
    Kei Yasuda, Hiroki Kawano and Yoshinobu Takakura
    
  • Analysis of Secretion Polarity after Gene Delivery to Epithelial Cells
    Masato Maruyama, Yoshihiko Watanabe and Yoshinobu Takakura
    
  • Characterizaton of Hepatic Delivery of Macromolecules with Large Volume Injection and its Therapeutic Application
    Naoki Kobayashi, Atsushi Kawase and Yoshinobu Takakura
    

• 29th Annual Meeting and Exposition of the Controlled Release Society, Seoul (Korea), July 20-25 (2002)

  • DNA Complexed with Cationic Liposomes Induces Inflammatory Cytokines from Cultured Mouse Macrophages
    Kei Yasuda, Yoshiyuki Ogawa, Michiyuki Kishimoto, Yasufumi Kaneda, Mitsuru Hashida and Yoshinobu Takakura
    
  • Induction of Antigen-specific Immune Responses Based on Chemical Modification of Antigen Proteins Targeting to Antigen Presenting Cells
    Yasuomi Yamasaki, Tomoko Ikenaga and Yoshinobu Takakura
    
  • Characterization of the Hydrodynamics-based Hepatic Delivery of Macromolecules
    Naoki Kobayashi, Shi Chen, Kiyoshi Yamaoka and Yoshinobu Takakura
    
  • Secretion Polarity and Intracellular Transport of Interferon-GFP Fusion Protein after Gene Delivery to Epithelial Cells
    Masato Maruyama, Toyokazu Yoshida, Yoshihiko Watanabe and Yoshinobu Takakura
    
  • Control of Disposition Characteristics of Plasmid DNA after Local Administration by Complexation with Cationic Macromolecules
    Atsushi Kawase, Naoki Kobayashi, Kiyoshi Yamaoka and Yoshinobu Takakura
    
  • Secretion Polarity after Erythropoietin Gene Transfer in Epithelial cells is Dependent on Gene Expression Mode
    Michiyuki Kishimoto, Yoshihiko Watanabe and Yoshinobu Takakura
    
  • Plasmid DNA Uptake and Subsequent Immune Responses in Dendritic Cells
    Takaharu Yoshinaga, Kei Yasuda and Yoshinobu Takakura
    

2000 年

• Millennial World Congress of Pharmaceutical Sciences, San Francisco (USA) 16-20 April (2000)

  • Plasmid DNA Uptake and Subsequent Activation in Cultured Mouse Peritoneal Macrophages
    Kei Yasuda, Miho Ueno, Aki Murao, Toshihide Takagi, Mitsuru Hashida, and Yoshinobu Takakura
    
  • Effects of Molecular Weight and Number of Negative Charges on Pharmacokinetics of Succinylated Proteins
    Yasuomi Yamasaki, Kazuya Sumimoto, Makiya Nishikawa, Fumiyoshi Yamashita, Kiyoshi Yamaoka, Mitsuru Hashida, and Yoshinobu Takakura
    
  • Characteristics of Hepatic Uptake of Plasmid DNA Related to Gene Expression and Immune Responses
    Naoki Kobayashi, Kiyoshi Yamaoka, Mitsuru Hashida, and Yoshinobu Takakura
    
  • Polarized Secretion of IFN- b in Epithelial Cells Following Gene Transfer with IFN- b Encoding Plasmid
    Masato Maruyama, Kiyo Nakanishi, Yoshihiko Watanabe, Mitsuru Hashida, and Yoshinobu Takakura
    

1999 年

• Strategies for Optimizing Oral Drug Delivery: Scientific to Regulatory Approaches, Kobe (Japan) 19-21 April (1999)

  • Transport Characteristics of a CYP3A4 Substrate Across Human Intestinal Caco-2 Cell Monolayers
    Yukiko Akazawa, Yuuichiro Imamura, Kazuteru Shimizu, Fumiyoshi Yamashita, Kiyoshi Yamaoka, Mitsuru Hashida and Yoshinobu Takakura