Culture Medium : 50 ml Neurobasal Medium 1 x B-27 supplement 0.5 mM glutamine 1/200 penicillin-streptomycin
Dish Coating :
Papain Solution(pH 7.4): 10-15 units/ml papain 0.45 mg/ml L-cystein hydrochrolide 0.01 % DNaseI in PBS
10)
Ref.: Free Radical Biology & Medicine, 28, 5, 665-672 (2000) J. Neurosci Res., 71, 811-818 (2003) J. Neurosci. Meth., 149, 110-120 (2005) 日薬理誌, 119, 163-166 (2002) 実験医学, 20, 15 (10月号, 2002) 脳・神経研究の進め方, 羊土社, 53-58