生体分子認識学分野

新着情報

○神経特異的な新規小胞体膜タンパク質の分子同定 (2006. 6)

Disturbance of cerebellar synaptic maturation in mutant mice lacking BSRPs,a novel brain-specific receptor-like protein family
(FEBS Lett. 580, 4057-4064, 2006.)
Taisuke Miyazaki, Kouichi Hashimoto, Atsushi Uda, Hiroyuki Sakagami, Yoshitaka Nakamura, Shin-ya Saito, Miyuki Nishi, Hideaki Kume, Akira Tohgo, Izumi Kaneko, Hisatake Kondo, Kohji Fukunaga, Masanobu Kano, Masahiko Watanabe and Hiroshi Takeshima

By DNA cloning, we have identified the BSRP (brain-specific receptor-like proteins) family of three members in mammalian genomes. BSRPs were predominantly expressed in the soma and dendrites of neurons and localized in the endoplasmic reticulum (ER). Expression levels of BSRPs seemed to fluctuate greatly during postnatal cerebellar maturation. Triple-knockout mice lacking BSRP members exhibited motor discoordination, and Purkinje cells (PCs) were often innervated by multiple climbing fibers with different neuronal origins in the mutant cerebellum. Moreover, the phosphorylation levels of protein kinase Ca (PKCa) were significantly down-regulated in the mutant cerebellum. Because cerebellar maturation and plasticity require metabotropic glutamate receptor signaling and resulting PKC activation, BSRPs are likely involved in ER functions supporting PKCa activation in PCs.