研究業績
高倉教授個人の業績へ
研究内容
・原著論文
・国際学会
・国内学会
・学位論文

原著論文

2022年
  • Development of allergic rhinitis immunotherapy using antigen-loaded small extracellular vesicles.
    Liu W, Ota M, Tabushi M, Takahashi Y, Takakura Y.
    J Control Release. 2022 Mar 14;345:433-442. PMID: 35301052
  • Development of hydrophobic interaction-based DNA supramolecules as efficient delivery carriers of CpG DNA to immune cells.
    Koichi Ito, Mustumi Kariya, Kento Yasui, Yuki Takahashi, Yoshinobu Takakura.
    J Pharm Sci. 2022;111(4):1133-1141. doi:10.1016/j.xphs.2021.11.027. PMID: 34871560
  • Enhanced Immunostimulatory Activity of Covalent DNA Dendrons.
    Kawamoto Y, Liu W, Yum J H, Park S, Sugiyama H, Takahashi Y, Takakura Y.
    ChemBioChem. 2022,23. doi:10.1002/cbic.202100583. PMID: 34881505

2021年
  • Interleukin-4-carrying small extracellular vesicles with a high potential as anti-inflammatory therapeutics based on modulation of macrophage function.
    Takenaka M, Yabuta A, Takahashi Y, Takakura Y.
    Biomaterials. 2021 Nov;278:121160. doi: 10.1016/j.biomaterials.2021.121160. Epub 2021 Oct 9. PMID: 34653934
  • Calcium Peroxide-Containing Polydimethylsiloxane-Based Microwells for Inhibiting Cell Death in Spheroids through Improved Oxygen Supply.
    Mizukami Y, Takahashi Y, Shimizu K, Konishi S, Takakura Y, Nishikawa M.
    Biol Pharm Bull. 2021;44(10):1458-1464. doi: 10.1248/bpb.b21-00269. PMID: 34602554
  • Combined use of chemically modified nucleobases and nanostructured DNA for enhanced immunostimulatory activity of CpG oligodeoxynucleotide.
    Araie Y, Ohtsuki S, Park S, Nagaoka M, Umemura K, Sugiyama H, Kusamori K, Takahashi Y, Takakura Y, Nishikawa M.
    Bioorg Med Chem. 2021;29:115864. doi:10.1016/j.bmc.2020.115864. PMID: 33223462
  • Critical contribution of macrophage scavenger receptor 1 to the uptake of nanostructured DNA by immune cells.
    Umemura K, Ohtsuki S, Nagaoka M, Kusamori K, Inoue T, Takahashi Y, Takakura Y, Nishikawa M.
    Nanomedicine. 2021;34:102386. doi:10.1016/j.nano.2021.102386. PMID: 33774131
  • Determining The Role of Surface Glycans in The Pharmacokinetics of Small Extracellular Vesicles.
    Yamamoto A, Yasue Y, Takahashi Y, Takakura Y.
    J Pharm Sci. 2021;110(9):3261-3267. doi:10.1016/j.xphs.2021.05.021. PMID: 34090899.
  • Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood.
    Matsumoto A, Takahashi Y, Ogata K, Kitamura S, Nakagawa N, Yamamoto A, Ishihama Y, Takakura Y.
    iScience. 2021;24(8):102839. doi:10.1016/j.isci.2021.102839. PMID:34368655.

2020年
  • Intracellular delivery o NF-κB inhibitor peptide utilizing small extracellular vesicles for the application of anti-inflammatory therapy.
    Takenaka M, Takahashi Y, Takakura Y.
    J Control Release. 2020;328:435-443. doi:10.1016/j.jconrel.2020.09.001. PMID: 32898592
  • Construction of Monomeric and Dimeric G-Quadruplex-Structured Cytosine-Phosphate-Guanine Oligonucleotides for Enhanced Uptake and Activation in Toll-Like Receptor 9-Positive macrophages.
    Liao W, Tan M, Kusamori K, Takakura Y, Nishikawa M.
    Nucleic Acid Ther. 2020. doi:10.1089/nat.2019.0843. Online ahead of print. PMID: 32559406
  • DNA density-dependent uptake of DNA origami-based two- or three-dimensional nanostructures by immune cells.
    Maezawa T, Ohtsuki S, Hidaka K, Sugiyama H, Endo M, Takahashi Y, Takakura Y, Nishikawa M.
    Nanoscale. 2020;12(27):14818-14824. doi:10.1039/d0nr02361b. PMID: 32633313.
  • Enhanced Immunostimulatory Activity of CpG Oligodeoxynucleotide by the Combination of Mannose Modification and Incorporation into Nanostructured DNA.
    Liao W, Akahira S, Hara RI, Wada T, Kusamori K, Takakura Y, Nishikawa M.
    Biol Pharm Bull. 2020;43(8):1188-1195. doi:10.1248/bpb.b20-00052. PMID: 32741939.
  • Analysis of tertiary structural features of branched DNA nanostructures with partially common sequences using small-angle X-ray scattering.
    Tan M, Takahashi N, Fujii S, Sakurai K, Kusamori K, Takahashi Y, Takakura Y, Nishikawa M.
    ACS Applied Bio Materials. 2020, 3, 308-314.
  • Therapeutic Application of Small Extracellular Vesicles (sEVs): Pharmaceutical and Pharmacokinetic Challenges.
    Takakura Y, Matsumoto A, Takahashi Y.
    Biol Pharm Bull. 2020;43:576-583. PMID: 32238700
  • Antitumor immunity by small extracellular vesicles collected from activated dendritic cells through effective induction of cellular and humoral immune responses.
    Matsumoto A, Asuka M, Takahashi Y, Takakura Y.
    Biomaterials. 2020;252:120112. PMID: 32422494
  • Development of CD40L-modified tumor small extracellular vesicles for the effective induction of anti-tumor immune response.
    Liu W, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.
    Nanomedicine (Lond). 2020 Jul;15(17):1641-1652. doi: 10.2217/nnm-2020-0071. PMID: 32664826
  • Incorporation of gelatin microspheres into HepG2 human hepatocyte spheroids for functional improvement through improved oxygen supply to spheroid core.
    Mizukami Y, Moriya A, Takahashi Y, Shimizu K, Konishi S, Takakura Y, Nishikawa M.
    Biol Pharm Bull. 2020;43(8):1220-1225. doi: 10.1248/bpb.b20-00141. PMID: 32741942
  • Development of RNA/DNA Hydrogel Targeting Toll-Like Receptor 7/8 for Sustained RNA Release and Potent Immune Activation.
    Komura F, Okuzumi K, Takahashi Y, Takakura Y, Nishikawa M.
    Molecules. 2020 Feb 7; 25(3):728. doi: 10.3390/molecules25030728. PMID: 32046113

2019年
  • Multifunctionalization of Cells with a Self-Assembling Molecule to Enhance Cell Engraftment.
    Takashima I, Kusamori K, Hakariya H, Takashima M, Vu TH, Mizukami Y, Noda N, Takayama Y, Katsuda Y, Sato SI, Takakura Y,Nishikawa M, Uesugi M.
    ACS Chem Biol. 2019 Apr 19; 14(4): 775-783.
  • Effects of Localization of Antigen Proteins in Antigen-Loaded Exosomes on Efficiency of Antigen Presentation.
    Arima Y, Liu W, Takahashi Y,Nishikawa M, Takakura Y.
    Mol Pharm. 2019 Jun 3; 16(6): 2309-2314.
  • Regulation of the distribution of cells in mixed spheroids by altering migration direction.
    Mizukami Y, Takahashi Y, Shimizu K, Konishi S, Takakura Y, Nishikawa M.
    Tissue Eng Part A. 2019 Mar; 25(5-6): 390-398
  • Development of a Nanostructured RNA/DNA Assembly as an Adjuvant Targeting Toll-Like Receptor 7/8.
    Komura F, Takahashi Y, Inoue I, Takakura Y, Nishikawa M.
    Nucleic Acid Ther. 2019 Dec; 29(6): 335-342
  • Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells.
    Matsumoto A, Takahashi Y, Ariizumi R, Nishikawa M, Takakura Y.
    Biomaterials. 2019 Dec; 225: 119518
  • Folding of single-stranded circular DNA into rigid rectangular DNA accelerates its cellular uptake.
    Ohtsuki S, Shiba Y, Maezawa T, Hidaka K, Sugiyama H, Endo M, Takahashi Y, Takakura Y, Nishikawa M.
    Nanoscale. 2019 Dec 28;11(48): 23416-23422
  • Blood concentrations of small extracellular vesicles are determined by a balance between abundant secretion and rapid clearance.
    Matsumoto A, Takahashi Y, Chang HY, Wu YW, Yamamoto A, Ishihama Y, Takakura Y.
    J Extracell Vesicles. 2019 Nov 26; 9(1): 1696517

2018年
  • Preservation of exosomes at room temperature using lyophilization.
    Charoenviriyakul C, Takahashi Y,Nishikawa M, Takakura Y.
    Int J Pharm. 2018, 553(1-2), 1-7.
  • Combined encapsulation of a tumor antigen and immune cells using a self-assembling immunostimulatory DNA hydrogel to enhance antigen-specific tumor immunity.
    Umeki Y, Saito M, Kusamori K, Tsujimura M, Nishimura M, Takahashi Y,Takakura Y, Nishikawa M.
    J Control Release. 2018, 288, 189-198.
  • Development of orally-deliverable DNA hydrogel by microemulsification and chitosan coating.
    Nomura D, Saito M, Takahashi Y, Takahashi Y, Takakura Y, Nishikawa M.
    Int J Pharm. 2018, 547, 556-562
    .
  • Possibility of Exosome-Based Therapeutics and Challenges in Production of Exosomes Eligible for Therapeutic Application.
    Yamashita T, Takahashi Y, Takakura Y.
    Biol Pharm Bull. 2018, 41, 835-842
    .
  • Enhanced Activity of Immunosuppressive Oligodeoxynucleotides by Incorporating Them into Hexapod-Like Nanostructured DNA.
    Araie Y, Takahashi Y, Takahashi Y, Takakura Y, Nishikawa M.
    Biol Pharm Bull. 2018, 41, 564-569
    .
  • Construction of nanostructured DNA harbouring phosphorodiamidate morpholino oligonucleotide for controlled tissue distribution in mice.
    Takahashi Y, Araie Y, Nomura D, Takahashi Y, Sano K, Saji H, Takakura Y, Nishikawa M.
    J Drug Target. 2018, 26, 373-381
    .
  • Role of extracellular vesicle surface proteins in the pharmacokinetics of extracellular vesicles.
    Charoenviriyakul C, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.
    Mol Pharm. 2018, 15, 1073-1080
    .

2017年
  • Control of polarization and tumoricidal activity of macrophages by multicellular spheroid formation.
    Tanaka Y, Nishikawa M, Mizukami Y, Kusamori K, Ogino Y, Nishimura S, Shimizu K, Konishi S, Takahashi Y, Takakura Y.
    J Control Release. 2017, 270, 177-183
    .
  • Safe and effective interferon-beta gene therapy for the treatment of multiple sclerosis by regulating biological activity through the design of interferon-beta-galectin-9 fusion proteins.
    Hamana A, Takahashi Y, Tanioka A, Nishikawa M, Takakura Y.
    Int J Pharm. 2017, 536, 310-317
    .
  • SELEX-Based Screening of Exosome-Tropic RNA.
    Yamashita T, Shinotsuka H, Takahashi Y, Kato K, Nishikawa M, Takakura Y.
    Biol Pharm Bull. 2017, 40, 2140-2145
    .
  • Reconstruction of Toll-like receptor 9-mediated responses in HEK-Blue hTLR9 cells by transfection of human macrophage scavenger receptor 1 gene.
    Ohtsuki S, Takahashi Y, Inoue T, Takakura Y, Nishikawa M.
    Sci Rep. 2017, 7, 13661
    .
  • Enhanced Class I Tumor Antigen Presentation via Cytosolic Delivery of Exosomal Cargos by Tumor-Cell-Derived Exosomes Displaying a pH-Sensitive Fusogenic Peptide.
    Morishita M, Takahashi Y, Nishikawa M, Ariizumi R, Takakura Y.
    Mol Pharm. 2017, 14, 4079-4086
    .
  • DNA nanotechnology-based composite-type gold nanoparticle-immunostimulatory DNA hydrogel for tumor photothermal immunotherapy.
    Yata T, Takahashi Y, Tan M, Nakatsuji H, Ohtsuki S, Murakami T, Imahori H, Umeki Y, Shiomi T, Takakura Y, Nishikawa M.
    Biomaterials. 2017, 146, 136-145
    .
  • Evaluation of antiviral effect of type I, II, and III interferons on direct-acting antiviral-resistant hepatitis C virus.
    Hamana A, Takahashi Y, Uchida T, Nishikawa M, Imamura M, Chayama K, Takakura Y.
    Antiviral Res. 2017, 146, 130-138
    .
  • Elucidation of the Mechanism of Increased Activity of Immunostimulatory DNA by the Formation of Polypod-like Structure.
    Mohri K, Nagata K, Ohtsuki S, Toyama S, Nonomura M, Takahashi Y, Takakura Y, Nishikawa M, Sakuma S.
    Pharm Res. 2017, 34, 2362-2370
    .
  • Retardation of Antigen Release from DNA Hydrogel Using Cholesterol-Modified DNA for Increased Antigen-Specific Immune Response.
    Umeki Y, Saito M, Takahashi Y, Takakura Y, Nishikawa M.
    Adv Healthc Mater. 2017, 6(20)
    .
  • Accelerated growth of B16BL6 tumor in mice through efficient uptake of their own exosomes by B16BL6 cells.
    Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.
    Cancer Sci. 2017, 108, 1803-1810
    .
  • Using size-controlled multicellular spheroids of murine adenocarcinoma cells to efficiently establish pulmonary tumors in mice.
    Nishikawa T, Tanaka Y, Kusamori K, Mizuno N, Mizukami Y, Ogino Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y, Nishikawa M.
    Biotechnol J. 2017, 12(8)
    .
  • In Vitro and In Vivo Stimulation of Toll-Like Receptor 9 by CpG Oligodeoxynucleotides Incorporated Into Polypod-Like DNA Nanostructures.
    Takahashi Y, Maezawa T, Araie Y, Takahashi Y, Takakura Y, Nishikawa M.
    J Pharm Sci. 2017, 106, 2457-2462.
  • Pharmacokinetics of Exosomes-An Important Factor for Elucidating the Biological Roles of Exosomes and for the Development of Exosome-Based Therapeutics.
    Morishita M, Takahashi Y, Nishikawa M, Takakura Y.
    J Pharm Sci. 2017, 106, 2265-2269
    .
  • Amelioration of Experimental Autoimmune Encephalomyelitis in Mice by Interferon-Beta Gene Therapy, Using a Long-Term Expression Plasmid Vector.
    Hamana A, Takahashi Y, Tanioka A, Nishikawa M, Takakura Y.
    Mol Pharm. 2017, 14, 1212-1217
    .
  • Optimization of Albumin Secretion and Metabolic Activity of Cytochrome P450 1A1 of Human Hepatoblastoma HepG2 Cells in Multicellular Spheroids by Controlling Spheroid Size.
    Nishikawa T, Tanaka Y, Nishikawa M, Ogino Y, Kusamori K, Mizuno N, Mizukami Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y.
    Biol Pharm Bull. 2017, 40, 334-338
    .
  • Targeted Delivery of Interferon Gamma Using a Recombinant Fusion Protein of a Fibrin Clot-Binding Peptide With Interferon Gamma for Cancer Gene Therapy.
    Ando M, Fujimoto M, Takahashi Y, Nishikawa M, Hamana A, Takakura Y.
    J Pharm Sci. 2017, 106, 892-897
    .
  • Improved sustained release of antigen from immunostimulatory DNA hydrogel by electrostatic interaction with chitosan.
    Ishii-Mizuno Y, Umeki Y, Onuki Y, Watanabe H, Takahashi Y, Takakura Y, Nishikawa M.
    Int J Pharm. 2017, 516, 392-400
    .
  • Cell type-specific and common characteristics of exosomes derived from mouse cell lines: Yield, physicochemical properties, and pharmacokinetics.
    Charoenviriyakul C,Takahashi Y, Morishita M, Matsumoto A, Nishikawa M, Takakura Y.

    Eur J Pharm Sci. 2017, 96, 316-322
    .
  • Role of Phosphatidylserine-Derived Negative Surface Charges in the Recognition and Uptake of Intravenously Injected B16BL6-Derived Exosomes by Macrophages.
    Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.

    J Pharm Sci. 2017, 106, 168-175
    .

2016年
  • Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA.
    Morishita M, Takahashi Y, Matsumoto A, Nishikawa M, Takakura Y.

    Biomaterials. 2016, 111, 55-65.
  • Depressive symptoms as a side effect of Interferon-α therapy induced by induction of indoleamine 2,3-dioxygenase 1.
    Murakami Y, Ishibashi T, Tomita E, Imamura Y, Tashiro T, Watcharanurak K, Nishikawa M, Takahashi Y, Takakura Y, Mitani S, Fujigaki H, Ohta Y, Kubo H, Mamiya T, Nabeshima T, Kim HC, Yamamoto Y, Saito K.

    Sci Rep. 2016,6:29920
    .
  • Polypod-shaped DNAs: Small Angle X-ray Scattering and Immunostimulatory Activity. (本論文の画像が表紙に採択されました)
    Sanada Y, Shiomi T, Okobira T, Tan M, Nishikawa M, Akiba I, Takakura Y, Sakurai K.

    Langmuir, 2016, 32, 3760-3765.
  • Effect of transgene expression level per cell in mice livers on induction of transgene-specific immune responses after hydrodynamic gene transfer.
    Yin Y, Takahashi Y, Hamana A, Nishikawa M, Takakura Y.

    Gene Therapy, 2016, 23, 565-571.
  • Analysis and Control of in Vivo Kinetics of Exosomes for the Development of Exosome-based DDS.
    Takahashi Y, Nishikawa M, Takakura Y.

    Yakugaku Zasshi, 2016, 136, 49-53.
  • Application of Magnesium Pyrophosphate-Based Sponge-Like Microparticles to Enhance the Delivery Efficiency and Adjuvant Effects of Polyriboinosinic-Polyribocytidylic Acid in Immune Cells.
    Takagi S, Takahashi Y, Sugimura K, Nishikawa M, Takakura Y.

    JPharm Sci. 2016, 105, 766-772.
  • Interferon-Inducible Mx Promoter-Driven, Long-Term Transgene Expression System of Interferon-β for Cancer Gene Therapy.
    Hamana A, Takahashi Y, Nishikawa M, Takakura Y.

    Hum Gene Ther. 2016; 27, 936-945
    .
  • Effect of exosome isolation methods on physicochemical properties of exosomes and clearance of exosomes from the blood circulation.
    Yamashita T, Takahashi Y, Nishikawa M, Takakura Y.

    Eur J Pharm Biopharm. 2016, 98,1-8.
  • Increased Insulin Secretion from Insuiln-Secreting Cells by Construction of Mixed Multicellular Spheroids. (本論文の画像が表紙に採択されました)
    Kusamori K, Nishikiawa M, Mizuno N, Nishikawa T, Masuzawa A, Tanaka Y, Mizukami Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y.

    Pharm Res. 2016, 33, 247-256. 
  • Self-assembling DNA hydrogel-based delivery of immunoinhibitory nucleic acids to immune cells.
    Nishida Y, Ohtsuki S, Araie Y, Umeki Y, Endo M, Emura T, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y, Nishikawa M.

    Nanomedicine: Nanotechnology, Biology, and Medicine.
    2016, 12, 123-130.

2015年

  • Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion.
    Tomoya Yata, Yuki Takahashi, Mengmeng Tan, Kumi Hidaka, Masayuki Endo, Hiroshi Sugiyama, Yoshinobu Takakura, Makiya Nishikawa.

    Sci Rep., 2015, 5, 14979
  • Atomic force microscopy analysis of orientation and bending of oligodeoxynucleotides in polypod-like structured DNA.
    Shiomi T, Tan M, Takahashi N, Endo M, Emura T, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y, Nishikawa M..

    Nano Research. 2015 Dec; 8(12): 3764-3771.
  • Contribution of Epigenetic Modifications to the Decline in Transgene Expression from Plasmid DNA in Mouse Liver.
    Zang L, Nishikawa M, Ando M, Takahashi Y, Takakura Y.

    Pharmaceutics. 2015 Aug; 7(3): 199-212.
  • Induction of Potent Antitumor Immunity by Sustained Release of Cationic Antigen from a DNA-Based Hydrogel with Adjuvant Activity.
    Umeki Y, Mohri K, Kawasaki Y, Watanabe H, Takahashi R, Takahashi Y, Takakura Y, Nishikawa M.

    Advanced Functional Materials. 2015, 25, 5758-67
  • Optimal Arrangement of Four Short DNA Strands for Delivery of Immunostimulatory Nucleic Acids to Immune Cells.
    Ohtsuki S, Matsuzaki N, Mohri K, Endo M, Emura T, Hidaka K, Sugiyama H, Takahashi Y, Ishiyama K, Kadowaki N, Takakura Y, Nishikawa M.

    Nucleic Acid Therapeutics. 2015, 25, 245-53
  • Molecular Design of Bisphosphonate-Modified Proteins for Efficient Bone Targeting In Vivo.
    Katsumi H, Sano J, Nishikawa M, Hanazawa K, Sakane T, Yamamoto A.

    PLoS One.. 2015, 10.
  • Self-assembling DNA dendrimer for effective delivery of immunostimulatory CpG DNA to immune cells.
    Mohri K, Kusuki E, Ohtsuki S, Takahashi N, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y, Nishikawa M.

    Biomacromolecules. 2015, 16, 1095-1101.
  • Quantitative Analysis of Tissue Distribution of the B16BL6-Derived Exosomes Using a Streptavidin-Lactadherin Fusion Protein and Iodine-125-Labeled Biotin Derivative After Intravenous Injection in Mice.
    Morishita M, Takahashi Y, Nishikawa M, Sano K, Kato K, Yamashita T, Imai T, Saji H, Takakura Y.

    J Pharm Sci. 2015 Feb;104(2):705-13.
  • Nasal delivery of Japanese cedar pollen Cryj1 by using self-gelling immunostimulatory DNA for effective induction of immune responses in mice.
    Ishii-Mizuno Y, Umeki Y, Takahashi Y, Kato Y, Takabayashi T, Fujieda S, Takakura Y, Nishikawa M.

    J Control Release. 2015 Feb 28;200:52-9.
  • Macrophage-dependent clearance of systemically administered B16BL6-derived exosomes from the blood circulation in mice.
    Imai T, Takahashi Y, Nishikawa M, Kato K, Morishita M, Yamashita T, Matsumoto A, Charoenviriyakul C, Takakura Y.

    J Extracell Vesicles. 2015 Feb 9;4:26238.

2014年
  • Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice.
    Kusamori K, Nishikawa M, Mizuno N, Nishikawa T,  Masuzawa A, Shimizu K,
    Konishi S, Takahashi Y, Takakura Y.
    J Control Release. 2014 Jan 10;173:119-24
  • Long-Term Elimination of Hepatitis C Virus from Human Hepatocyte Chimeric Mice After Interferon-γ Gene Transfer.
    Takahashi Y, Ando M, Nishikawa M, Hiraga N, Imamura M, Chayama K, Takakura Y.
    Hum Gene Ther Clin Dev. 2014 Mar;25(1):28-39
  • Efficient delivery of immunostimulatory DNA to mouse and human immune cells through the construction of polypod-like structured DNA.
    Uno S, Nishikawa M, Mohri K, Umeki Y, Matsuzaki N, Takahashi Y, Fujita H, Kadowaki N, Takakura Y.
    Nanomedicine. 2014 May;10(4):765-74.
  • Injectable, self-gelling, biodegradable, and immunomodulatory DNA hydrogel for antigen delivery.
    Nishikawa M, Ogawa K, Umeki Y, Mohri K, Kawasaki Y, Watanabe H, Takahashi N, Kusuki E, Takahashi R, Takahashi Y, Takakura Y.
    J Control Release. 2014 Apr 28;180:25-32.
  • DNA nanotechnology-based development of delivery systems for bioactive compounds.
    Mohri K, Nishikawa M, Takahashi Y, Takakura Y.
    Eur J Pharm Sci. 2014 Jul 16;58:26-33.
  • Enhancement of anticancer effect of interferon-γ gene transfer against interferon-γ-resistant tumor by depletion of tumor-associated macrophages.
    Kiyota T, Takahashi Y, Watcharanurak K, Nishikawa M, Ohara S, Ando M, Watanabe Y, Takakura Y
    Mol Pharm. 2014 May 5;11(5):1542-9.

  • Removal of transgene-expressing cells by a specific immune response induced by sustained transgene expression.
    Yin Y, Takahashi Y, Ebisuura N, Nishikawa M, Takakura Y.
    J Gene Med. 2014 Mar-Apr;16(3-4):97-106.

  • Effects of upregulated indoleamine 2, 3-dioxygenase 1 by interferon γ gene transfer on interferon γ-mediated antitumor activity.
    Watcharanurak K, Zang L, Nishikawa M, Yoshinaga K, Yamamoto Y, Takahashi Y, Ando M, Saito K, Watanabe Y, Takakura Y.

    Gene Ther. 2014 Jun 12.

  • X-ray scattering from immunostimulatory tetrapod-shaped DNA in aqueous solution to explore its biological activity-conformation relationship.
    Sanada Y, Sakamoto S, Shiomi T, Okobira T, Mylonas E, Ohta N, Yagi N, Nishikawa M, Akiba I, Takakura Y, Sakurai K.

    J Phys Chem B. 2014 Sep 4;118(35):10373-9.
  • Synthetic Molecules that Protect Cells from Anoikis and Their Use in Cell Transplantation.
    Frisco-Cabanos HL, Watanabe M, Okumura N, Kusamori K, Takemoto N, Takaya J, Sato S, Yamazoe S, Takakura Y, Kinoshita S, Nishikawa M, Koizumi N, Uesugi M.

    Angew Chem Int Ed Enql. 2014 Oct 13;53(42):11208-13.
  • Prevention of adverse events of interferon γ gene therapy by gene
    delivery of interferon γ-heparin-binding domain fusion protein
    in mice.
    Ando M, Takahashi Y, Yamashita T, Fujimoto M, Nishikawa M, Watanabe Y, Takakura Y.

    Molecular Therapy ? Methods & Clinical Development . 2014 1, 14023


2013年

  • Regulation of immunological balance by sustained interferon-γ gene transfer for acute phase of atopic dermatitis in mice.
    Watcharanurak K, Nishikawa M, Takahashi Y, Kabashima K,
    Takahashi R, Takakura Y.
    Gene Ther. 2013 May;20(5):538-44
  • Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection.
    Takahashi Y, Nishikawa M, Shinotsuka H, Matsui Y, Ohara S, Imai T, Takakura Y.
    J Biotechnol. 2013 May 20;165(2):77-84
  • Poly(N-isopropylacrylamide)-coated microwell arrays for construction and recovery of multicellular spheroids.
    Shimizu K, Kusamori K, Nishikawa M, Mizuno N, Nishikawa T, Masuzawa A,
    Katano S, Takahashi Y, Takakura Y, Konishi S.
    J Biosci Bioeng. 2013 Jun;115(6):695-9
  • Small-molecule-induced clustering of heparan sulfate promotes cell adhesion.
    Takemoto N, Suehara T, Frisco H, Sato S, Sezaki T, Kusamori K, Kawazoe Y, Park SM, Yamazoe S, Mizuhata Y, Inoue R, Miller Gavin, Hansen S, Jayson G, Gardiner J, Kanaya T, Tokitoh N, Ueda K, Takakura Y, Kioka N, Nishikawa M, Uesugi M.
    J Am Chem Soc. 2013 Jul 31;135(30):11032-9
  • Gene delivery of albumin binding peptide-interferon-gamma fusion protein with improved pharmacokinetic properties and sustained biological activity.
    Miyakawa N, Nishikawa M, Takahashi Y, Ando M,
    Misaka M, Watanabe Y, Takakura Y.
    J Pharm Sci. 2013 Sep;102(9):3110-8
  • Expression profile-dependent improvement of insulin sensitivity by gene delivery of interleukin-6 in a mouse model of type II diabetes.
    Mukumoto H, Takahashi Y, Ando M, Nishikawa M, Takakura Y.
    Mol Pharm. 2013 Oct 7;10(10):3812-21

2012年
  • Development of safe and effective nonviral gene therapy by eliminating CpG motifs from plasmid DNA vector.
    Takahashi Y, Nishikawa M, Takakura Y.
    Front Biosci (Schol Ed). 2012 Jan 1;4:133-41.
  • Comparison of antigen expression from plasmid DNA in tumor-free and antigen-expressing tumor-bearing mice.
    Guan X, Nishikawa M, Li H, Takahashi Y, Takakura Y.
    Hum Vaccin Immunother. 2012 Feb;8(2):194-200.
  • Constant and steady transgene expression of interferon-γ by optimization of plasmid construct for safe and effective interferon-γ gene therapy.
    Ando M, Takahashi Y, Nishikawa M, Watanabe Y, Takakura Y.
    J Gene Med. 2012 Apr;14(4):288-95
  • Near-infrared fluorescence probes for enzymes based on binding affinity modulation of squarylium dye scaffold.
    Oushiki D, Kojima H, Takahashi Y, Komatsu T, Terai T, Hanaoka K, Nishikawa M, Takakura Y, Nagano T.
    Anal Chem. 2012 May 15;84(10):4404-10
  • Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells.
    Mohri K, Nishikawa M, Takahashi N, Shiomi T, Matsuoka N, Ogawa K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y.
    ACS Nano. 2012 Jul 24;6(7):5931-40
  • Self-assembling CpG DNA nanoparticles for efficient antigen delivery and immunostimulation.
    Rattanakiat S, Nishikawa M, Takakura Y.
    Eur J Pharm Sci. 2012 Sep 29;47(2):352-8
  • Fibronectin inhibits cytokine production induced by CpG DNA in macrophages without direct binding to DNA.
    Yoshida H, Nishikawa M, Yasuda S, Toyota H, Kiyota T, Takahashi Y, Takakura Y.
    Cytokine. 2012 Oct;60(1):162-70
  • Increased immunostimulatory activity of polypod-like structured DNA by ligation of the terminal loop structures.
    Mohri K, Takahashi N, Nishikawa M, Kusuki E, Shiomi T, Takahashi Y, Takakura Y.
    J Control Release. 2012 Nov 10;163(3):285-92
  • Controlling the kinetics of interferon transgene expression for improved gene therapy.
    Watcharanurak K, Nishikawa M, Takahashi Y, Takakura Y.
    J Drug Target. 2012 Nov;20(9):764-9
  • Control of spatiotemporal distribution of interferon γ by genetically fusing functional peptides.
    Ando M, Takahashi Y, Nishikawa M, Takakura Y.
    Yakugaku Zasshi. 2012;132(12):1399-406

2011年

  • Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice.
    Nishizaki C, Nishikawa M, Yata T, Yamada T, Takahashi Y, Oku M, Yurimoto H, Sakai Y, Nakanishi K, Takakura Y. Free Radic Biol Med., 51(3):773-9 (2011)
  • Increase in CpG DNA-induced inflammatory responses by DNA oxidation in macrophages and mice.
    Yoshida H, Nishikawa M, Kiyota T, Toyota H, Takakura Y. Free Radic Biol Med., 51(2):424-31 (2011)
  • Saturation of transgene protein synthesis from mRNA in cells producing a large number of transgene mRNA.
     Takahashi Y, Nishikawa M, Takiguchi N, Suehara T, Takakura Y. Biotechnol Bioeng., in press
  • Nucleic acid drug delivery and targeting.
    Takakura Y. Pharm Res., 28(4):691-3 (2011)
  • 5'-Phosphate oligodeoxynucleotides enhance the phosphodiester-CpG DNA-induced inflammatory response in macrophages.
    Yoshida H, Nishikawa M, Kiyota T, Uno S, Toyota H, Takahashi R, Narita M, Takakura Y. Eur J Immunol., 41(2):425-36 (2011)
  • Prolonged circulation half-life of interferon γ activity by gene delivery of interferon γ-serum albumin fusion protein in mice.
    Miyakawa N, Nishikawa M, Takahashi Y, Ando M, Misaka M, Watanabe Y, Takakura Y. J Pharm Sci., 100(6):2350-7 (2011)
  • Inhibition of nuclear delivery of plasmid DNA and transcription by interferon γ: hurdles to be overcome for sustained gene therapy.
    Zang L, Nishikawa M, Machida K, Ando M, Takahashi Y, Watanabe Y, Takakura Y. Gene Ther., 18(9):891-7 (2011)

2010年


  • Persistent interferon transgene expression by RNA interference-mediated silencing of interferon receptors.
    Takahashi Y, Vikman E, Nishikawa M, Ando M, Watanabe Y, Takakura Y.
    J Gene Med. 2010 Sep; 12(9): 739-746

  • Sustained exogenous expression of therapeutic levels of IFN-gamma ameliorates atopic dermatitis in NC/Nga mice via Th1 polarization.
    Hattori K, Nishikawa M, Watcharanurak K, Ikoma A, Kabashima K, Toyota H, Takahashi Y, Takahashi R, Watanabe Y, Takakura Y.
     J Immunol. 2010 Mar;184(5), 2729-2735
  • Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination.
     Guan X, Nishikawa M, Takemoto S, Ohno Y, Yata T, Takakura Y.
    J Gene Med. 2010 Mar;12(3), 301-309
  • Comparison of the type of liposome involving cytokine production induced by non-CpG Lipoplex in macrophages.
    Yasuda S, Yoshida H, Nishikawa M, Takakura Y.
    Mol Pharm. 2010 Apr 5;7(2), 533-542
  • Development of a novel Hsp70-based DNA vaccine as a multifunctional antigen delivery system.
    Yamaoka A, Guan X, Takemoto S, Nishikawa M, Takakura Y.
    J Control Release. 2010 Mar 19;142(3), 411-415
  • Bootstrap method-based estimation of the minimum sample number for obtaining pharmacokinetic parameters in preclinical experiments.Takemoto S, Yamaoka K, Nishikawa M, Yano Y, Takakura Y.
    J Pharm Sci. 2010 Apr;99(4), 2176-2184

2009 年

  • Control of hypoxia-induced tumor cell adhesion by cytophilic human catalase.
    Yata T, Nishikawa M, Nishizaki C, Oku M, Yurimoto H, Sakai Y, Takakura Y.
    Free Radic Biol Med. 2009 Dec 15;47(12):1772-8. Epub 2009 Oct 3. Abstract (PubMed)
  • Induction of tumor-specific immune response by gene transfer of Hsp70-cell penetrating peptide fusion protein to tumors in mice
    Makiya Nishikawa, Takayuki Otsuki, Atsushi Ota, Xin Guan, Seiji Takemoto, Yuki Takahashi and Yoshinobu Takakura
    Mol Ther. 2009 Sep 1. [Epub ahead of print] Abstract (PubMed)
  • The assembly of a short linear natural cytosine-phosphate-guanine DNA into dendritic structures and its effect on immunostimulatory activity
    Sakulrat Rattanakiat, Makiya Nishikawa, Hisakage Funabashi, Dan Luo and Yoshinobu Takakura
    Biomaterials. 2009 Oct;30(29):5701-6. Abstract (PubMed)
  • Effects of methyl-β-cyclodextrin treatment on secretion profile of interferon-β and ZO-1 architecture in MDCK cell monolayers
    Masato Maruyama, Kayo Ishida, Yoshihiko Watanabe, Makiya Nishikawa and Yoshinobu Takakura
    Biol Pharm Bull. 2009 May;32(5):910-5.Abstract (PubMed)
  • TLR9-depdendent systemic interferon-beta production by intravenous injection of plasmid DNA/cationic liposome complex in mice
    Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda, Yumiko Mizuno, Hiroyasu Toyota, Tsuyoshi Kiyota, Rei Takahashi and Yoshinobu Takakura
    J Gene Med. 2009 Aug;11(8):708-17. Abstract (PubMed)
  • Nonviral vector-mediated RNA interference: its gene silencing characteristics and important factors to achieve RNAi-based gene therapy
    Yuki Takahashi, Makiya Nishikawa and Yoshinobu Takakura
    Adv Drug Deliv Rev. 2009 Jul 25;61(9):760-6. Abstract (PubMed)
  • Enhanced CTL response by controlled intracellular trafficking of antigen in dendritic cells following DNA vaccination
    Keiko Isaji, Atsushi Kawase, Mitsuhiro Matono, Xin Guan, Makiya Nishikawa and Yoshinobu Takakura
    J Control Release. 2009 May 5;135(3):227-33. Abstract (PubMed)
  • Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on sustainability of transgene expression
    Masaru Mitsui, Makiya Nishikawa, Lei Zang, Mitsuru Ando, Kayoko Hattori, Yuki Takahashi, Yoshihiko Watanabe and Yoshinobu Takakura
    J Gene Med. 2009 May;11(5):435-43. Abstract (PubMed)
  • Catalase delivery for inhibiting ROS-mediated tissue injury and tumor metastasis
    Makiya Nishikawa, Mitsuru Hashida, Yoshinobu Takakura
    Adv Drug Deliv Rev. 2009 Apr 28;61(4):319-26. Abstract (PubMed)

2008 年

  • Reactivation of Silenced Transgene Expression in Mouse Liver by Rapid, Large-volume Injection of Isotonic Solution.
    Makiya Nishikawa, Ayumi Nakayama, Yuki Takahashi, Yasushi Fukuhara and Yoshinobu Takakura
    Hum Gene Ther. 2008 Oct;19(10):1009-20. Abstract (PubMed)
  • Gene silencing of beta-catenin in melanoma cells retards their growth but promotes the formation of pulmonary metastasis in mice.
    Yuki Takahashi, Makiya Nishikawa, Tetsuya Suehara, Naomi Takiguchi and Yoshinobu Takakura
    Int J Cancer. 2008 Nov 15;123(10):2315-20. Abstract (PubMed)
  • Unique cytokine production profile following stimulation with DNA in macrophages from NZB/W F1 mice
    Yoshiyuki Ogawa, Takaharu Yoshinaga, Makiya Nishikawa and Yoshinobu Takakura
    Biol Pharm Bull. 2008 Jun;31(6):1244-9. Abstract (PubMed)
  • Quantitative and temporal analysis of gene silencing in tumor cells induced by small interfering RNA or short hairpin RNA expressed from plasmid vectors
    Yuki Takahashi, Kiyoshi Yamaoka, Makiya Nishikawa and Yoshinobu Takakura
    J Pharm Sci. 2009 Jan;98(1):74-80. Abstract (PubMed)
  • Inhibition of tumor cell growth in the liver by RNA interference-mediated suppression of HIF-1α expression in tumor cells and hepatocytes
    Yuki Takahashi, Makiya Nishikawa and Yoshinobu Takakura
    Gene Ther. 2008 Apr;15(8):572-82. Abstract (PubMed)
  • Cellular activation by plasmid DNA in various macrophages in primary culture
    Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda, Yumiko Mizuno and Yoshinobu Takakura
    J Pharm Sci. 2008 Oct;97(10):4575-85. Abstract (PubMed)
  • Enhanced immunostimulatory activity of oligonucleotides by Y-shape formation
    Makiya Nishikawa, Mitsuhiro Matono, Sakulrat Rattanakiat, Nao Matsuoka and Yoshinobu Takakura
    Immunology. 2008 Jun;124(2):247-55.Abstract (PubMed)
2007 年
  • Effects of inflammatory response on in vivo transgene expression by plasmid DNA in mice
    Keiko Kako, Makiya Nishikawa, Hiroyuki Yoshida and Yoshinobu Takakura
    J Pharm Sci. 2007 Dec 6;97(8):3074-3083. Abstract (PubMed)
  • Hsp-based tumor vaccines: state-of-the-art and future directions
    Yoshinobu Takakura, Seiji Takemoto and Makiya Nishikawa.
    Curr Opin Mol Ther. 2007 Aug;9(4):385-91. Abstract (PubMed)
  • Improved anti-cancer effect of interferon gene transfer by sustained expression using CpG-reduced plasmid DNA
    Hiroki Kawano, Makiya Nishikawa, Masaru Mitsui, Yuki Takahashi, Keiko Kako, Kiyoshi Yamaoka, Yoshihiko Watanabe and Yoshinobu Takakura
    International
    journal of Cancer. 2007 Jul 15;121 (2):401-6. Abstract (PubMed)
  • Plasmid DNA uptake and subsequent cellular activation characteristics in human monocyte-derived cells in primary culture
    Yuga Fukuhara, Tomoyuki Naoi, Yoshiyuki Ogawa, Makiya Nishikawa and Yoshinobu Takakura
    Journal of Pharmaceutical Sciences. 2007 Jun;96 (6):1576-84.
    Abstract (PubMed)
  • Design of PCR-amplified DNA fragments for in vivo gene delivery: size-dependency on stability and transgene expression
    Kazuhiro Hirata, Makiya Nishikawa, Naoki Kobayashi, Yuki Takahashi and Yoshinobu Takakura
    Journal of Pharmaceutical Sciences. 2007 Mar 23; in press. Abstract (PubMed)
  • DNA and its cationic lipid complexes induce CpG motif-dependent activation of murine dendritic cells
    Takaharu Yoshinaga, Kei Yasuda, Yoshiyuki Ogawa, Makiya Nishikawa and Yoshinobu Takakura
    Immunology. 2007 Mar;120 (3):295-302. Abstract (PubMed)
  • Induction of antigen-specific cytotoxic T lymphocytes by immunization with negatively charged soluble antigen through scavenger receptor-mediated delivery
    Yasu
    omi Yamasaki, Tomoko Ikenaga, Takayuki Otsuki, Makiya Nishikawa and Yoshinobu Takakura
    Vaccine. 2007 Jan 2;2
    5(1):85-91. Abstract (PubMed)

2006 年

  • Histgram analysis of pharmacokinetic parameters by bootstrap resampling from one-point data in animal experiments
    Seiji Takemoto, Kiyoshi Yamaoka, Makiya Nishikawa and Yoshinobu Takakura
    Drug Metabolism and Pharmacokinetics. 2006 Dec;21 (6):458-64.
    Abstract (PubMed)
  • Suppression of tumor growth by intratumoral injection of short hairpin RNA-expressing plasmid DNA targeting beta-catenin or hypoxia-inducible factor 1alpha
    Yuki Takahashi, Makiya Nishikawa and Yoshinobu Takakura
    J Control Release. 2006 Nov 10;116 (1):90-5. Abstract (PubMed)
  • Enhanced antigen-specific antibody production following polyplex-based DNA vaccination via the intradermal route in mice
    Atsushi Kawase, Keiko Isaji, Ayumi Yamaoka, Naoki Kobayashi, Makiya Nishikawa and Yoshinobu Takakura
    Vaccine. 2006 Jul 7;24 (27-28):5535-45. Abstract (PubMed)
  • Moment analysis for kinetics of gene silencing by RNA interference
    Yuki Takahashi, Kiyoshi Yamaoka, Makiya Nishikawa and Yoshinobu Takakura
    Biotechnol Bioeng. 2006 Mar 5;93 (4):816-9. Abstract (PubMed)

2005 年

  • Cellular uptake and activation characteristics of naked plasmid DNA and its cationic liposome complex in human macrophages
    Ikuko Yamane, Makiya Nishikawa and Yoshinobu Takakura
    Int J Pharm. 2005 Nov 23;305 (1-2):145-53. Abstract (PubMed)
  • Sequence-specific suppression of mdr1a/1b expression in mice via RNA interference
    Yumi Matsui, Naoki Kobayashi, Makiya Nishikawa and Yoshinobu Takakura
    Pharm Res. 2005 Dec;22 (12):2091-8. Abstract (PubMed)
  • The uptake and degradation of DNA is impaired in macrophages and dendritic cells from NZB/W F(1) mice
    Yoshiyuki Ogawa, Takaharu Yoshinaga, Kei Yasuda, Makiya Nishikawa and Yoshinobu Takakura
    Immunol Lett. 2005 Oct 15;101 (1):32-40. Abstract (PubMed)
  • Gene Silencing in primary and metastatic tumors bby small interfering RNA delivery in mice: quantitative analysis using melanoma cells expressing firefly and sea pansy luciferases
    Yuki Takahashi, Makiya Nishikawa, Naoki Kobayashi and Yoshinobu Takakura
    J Control Release. 2005 Jul 20;105 (3):332-43.
    Abstract (PubMed)
  • Cholesterol is required for the polarized secretion of erythropoietin in Madin-Darby canine kidney cells
    Masato Maruyama, Michiyuki Kishimoto, Kayo Ishida, Yoshihiko Watanabe, Makiya Nishikawa, S. Masuda, R. Sasaki and Yoshinobu Takakura
    Arch Biochem Biophys. 2005 Jun 15;438 (2):174-81. Abstract (PubMed)
  • Pharmacokinetic and tissue distribution mechanism of mouse recombinant heat shock protein 70 in mice
    Seiji Takemoto, Makiya Nishikawa and Yoshinobu Takakura
    Pharm Res. 2005 Mar ;22 (3):419-26. Abstract (PubMed)
  • Manipulation of local disposition and gene expression characteristics of plasmid DNA following intramuscular administration by complexation with cationic macromolecule
    Atsushi Kawase, Naoki Kobayashi, Keiko Isaji, Makiya Nishikawa and Yoshinobu Takakura
    Int J Pharm. 2005 Apr 11;293 (1-2):291-301. Abstract (PubMed)
  • Permeability modulation of human intestinal Caco-2 cell monolayers by interferons
    Hiroko Kawaguchi, Yukiko Akazawa, Yoshihiko Watanabe and Yoshinobu Takakura
    Eur J Pharm Biopharm. 2005 Jan;59 (1):45-50. Abstract (PubMed)
  • Macrophage activation by a DNA/cationic liposome complex requires endosomal acidification and TLR9-dependent and -independent pathways
    Kei Yasuda, Yoshiyuki Ogawa, Ikuko Yamane, Makiya Nishikawa and Yoshinobu Takakura
    J Leukoc Biol. 2005 Jan;77 (1):71. Abstract (PubMed)

2004 年

  • Analysis methods and recent advances in nonlinear pharmacokinetics from in vitro through in loci to in vivo
    Kiyoshi Yamaoka and Yoshinobu Takakura
    Drug Metab Pharmacokinet. 2004 Dec;19 (6):397-406 Abstract (PubMed)
  • Secretion Mode and Subcellular Localization of Human Interferon- b Exogenously Expressed in Porcine Renal Epithelial LLC-PK 1 Cells
    Teruko Nishio, Masato Maruyama, Toyokazu Yoshida, Yoshihiko Watanabe, Makiya Nishikawa and Yoshinobu Takakura
    Biol Pharm Bull. 2004 Oct;27 (10):1653. Abstract (PubMed)
  • Simultaneous Detection of DsRed2-tagged and EGFP-tagged Human b -Interferons in the Same Single Cells
    Masato Maruyama, Teruko Nishio, Toyokazu Yoshida, Chisaki Ishida, Kayo Ishida, Yoshihiko Watanabe, Makiya Nishikawa and Yoshinobu Takakura
    J Cell Biochem. 2004 Sep 15;93 (3):497. Abstract (PubMed)
  • Significant Role of Liver Sinusoidal Endothelial Cells in Hepatic Uptake and Degradation of Naked Plasmid DNA following Intravenous Injection
    Jin Hisazumi, Naoki Kobayashi, Makiya Nishikawa and Yoshinobu Takakura
    Pharm Res. 2004 Jul;21 (7):1223-1228. Abstract (PubMed)
  • Subcellular Trafficking of Exogenously Expressed Interferon- b in Madin-Darby Canine Kidney Cells
    Masato Maruyama, Teruko Nishio, Takako Kato, Toyokaza Yoshida, Chisaki Ishida, Yoshihiko Watanabe, Makiya Nishikawa, Yasufumi Kaneda and Yoshinobu Takakura
    J Cell Physiol. 2004 Oct;201 (1):117-25. Abstract (PubMed)
  • Induction of Cytotoxic T Lymphocytes following Immunization with Cationized Soluble Antigen
    Tomoko Ikenaga, Yasuomi Yamasaki, Kohsuke Shakushiro, Makiya Nishikawa and Yoshinobu Takakura
    Vaccine. 2004 Jun 30;22 (20):2609-16. Abstract (PubMed)
  • Efficient scavenger receptor-mediated uptake and cross-presentation of negatively charged soluble antigens by dendritic cells
    Kohsuke Shakushiro, Yasuomi Yamasaki, Makiya Nishikawa and Yoshinobu Takakura
    Immunology. 2004 Jun;112 (2):211-8. Abstract (PubMed)
  • Hydrodynamic-based Procedure Invovlves Transient Hyperpermeability in the Hepatic Cellular Membrane: Implication of a Nonspecific Process in Efficient Intracellular Gene Delivery
    Naoki Kobayashi, Makiya Nishikawa, Kazauhiro Hirata and Yoshinobu Takakura
    J Gene Med. 2004 May;6 (5):584-92. Abstract (PubMed)
  • Hepatic Delivery of Particulates in the Submicron Range by a Hydrodynamic-based Procedure: Implications for Particulate Gene Delivery Systems
    Naoki Kobayashi, Kazuhiro Hirata, Shi Chen, Atsushi Kawase, Makiya Nishikawa and Yoshinobu Takakura
    J Gene Med. 2004 Apr;6 (4):455-63. Abstract (PubMed)
  • Restricted Cytokine Production from Mouse Peritoneal Macrophages in Culture in spite of Extensive Uptake of Plasmid DNA
    Kei Yasuda, Hiroki Kawano, Ikuko Yamane, Yoshiyuki Ogawa, Takaharu Yoshinaga, Makiya Nishikawa and Yoshinobu Takakura
    Immunology. 2004 Mar;111 (3):282-90. Abstract (PubMed)
  • An Evaluation Method for Nonlinear Local Disposition in Rat Liver and Kidney
    Takako Kato, Kiyoshi Yamaoka and Yoshinobu Takakura
    Drug Metab Dispos. 2004 Feb;32(2):230-4. Abstract (PubMed)
  • Vector-based In Vivo RNA Interference : Dose- and Time- Dependent Suppression of Transgene Expression
    Naoki Kobayashi, Yumi Matsui, Atsushi Kawase, Kazuhiro Hirata, Makoto Miyagishi, Makiya Nishikawa and Yoshinobu Takakura
    J Pharmacol Exp Ther.2004, Feb;308 (2):688-93. Abstract (PubMed)
  • Effect of interferon g on the Pharmacokinetics of Digoxin, a P-glycoprotein Substrate, Intravenously Injected into the Mice
    Hiroko Kawaguchi, Yumi Matsui, Yoshihiko Watanabe and Yoshinobu Takakura
    J Pharmacol Exp Ther. 2004 Jan;308 (1):91-6. Abstract (PubMed)

2003 年

  • Disposition and Gene Expression Characteristics in Solid Tumors and Skeletal Muscle after Directe Injection of Naked Plasmid DNA in Mice
    Atsushi Kawase, Takehiko Nomura, Kei Yasuda, Naoki Kobayashi, Mitsuru Hashida and Yoshinobu Takakura
    J. Pharm. Shi. 2003 Jun;92 (6):1295-304. Abstract (PubMed)
  • Efficient scavenger receptor-mediated hepatic targeting of proteins by introduction of negative charges on the proteins by aconitylation : the influence of charge density and size of the proteins molecules
    Yasuomi Yamasaki, Jin Hisazumi, Kiyoshi Yamaoka and Yoshinobu Takakura
    Eur. J. Pharm. Shi. 2003 Apr;18 (5):305-12. Abstract (PubMed)

2002 年

  • Therapeutic Effect of intravenous interferon gene delivery with naked plasmid DNA in murine metastasis models
    Naoki Kobayashi, Takeshi Kuramoto, Shi Chen, Yoshihiko Watanabe and Yoshinobu Takakura
    Mol Ther. 2002 Dec;6 (6):737-44. Abstract (PubMed)
  • Efficient uptke and rapid degradation of plasmid DNA by murine dendritic cells via a specific mechanism
    Takaharu Yoshinaga, Kei Yasuda, Yoshiyuki Ogawa, and Yoshinobu Takakura
    Biochem. Biophys. Res. Commun. 2002 Dec;299 (3):389-94. Abstract (PubMed)
  • Effect of interferons on P-glycoprotein-mediated rhodamine-123 efflux in cultured rat hepatocytes
    Yukiko Akazawa, Hiroko Kawaguchi, Maki Funahashi, Yoshihiko Watanabe, Kiyoshi Yamaoka, Mitsuru Hashida and Yoshinobu Takakura
    J. Pharm. Sci.2002 Oct;91 (10):2110-5. Abstract (PubMed)
  • Plasmid DNA Activates Murine Macrophages to Induce Inflammatory Cytokines in a CpG Motif-Independent Manner by Complex Formation with Cationic Liposomes
    Kei Yasuda, Yoshiyuki Ogawa, Michiyuki Kishimoto, Toshihide Takagi, Mitsuru Hashida, and Yoshinobu Takakura
    Biochem. Biophys. Res. Commun. 2002 Apr;293 (1):344-8. Abstract (PubMed)
  • Pharmacokinetic Analysis of In Vivo Disposition of Succinylated Proteins Targeted to Liver Non-parenchymal Cells via Scavenger Receptors: Importance of Molecular Size and Negative Charge Density for In Vivo Recognition by Receptors
    Yasuomi Yamasaki, Kazuya Sumimoto, Makiya Nishikawa, Fumiyoshi Yamashita, Kiyoshi Yamaoka, Mitsuru Hashida and Yoshinobu Takakura
    J. Pharmacol. Exp. Ther. 2002 May;301 (2):467-77. Absract (PubMed)

2001 年

  • Hepatic Uptake and Gene Expression Mechanisms following Intravenous Administration of Plasmid DNA by Conventional and Hydrodynamics-Based Procedures
    Naoki Kobayashi, Takeshi Kuramoto Kiyoshi Yamaoka, Mitsuru Hashida, and Yoshinobu Takakura
    J. Pharmacol. Exp. Ther. 2001 Jun;297 (3):853-60. Abstract (PubMed)
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