Research Project for Practical Drug Discovery


Laboratory of Biopharmaceutical Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University
Laboratory of Advanced Biopharmaceuticals, National Institute of Biomedical Innovation, Health and Nutrition


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Original research articles from this laboratory / Akiba’s publication


  • Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Ito T, Minamitani T, Hayakawa M, Otsubo R, Akiba H, Tsumoto K, Matsumoto M, Yasui T
    Sci. Rep. 11, 22341 (2021).
  • Production of IgG1-based bispecific antibody without extra cysteine residue via intein-mediated protein trans-splicing
    Akiba H, Ise T, Nagata S, Kamada H, Ohno H, Tsumoto K
    Sci. Rep. 11, 19411 (2021).
  • Epitope-dependent thermodynamic signature of single-domain antibodies against hen egg lysozyme
    Akiba H, Tamura H, Caaveiro JMM, Tsumoto K
    J. Biochem. accepted.
  • Novel neutralizing human monoclonal antibodies against tetanus neurotoxin
    Minamitani T, Kiyose K, Otsubo R, Ito T, Akiba H, Furuta RA, Inoue T, Tsumoto K, Satake M, Yasui T
    Sci. Rep. 11, 12134 (2021).


  • The Kg-antigen, RhAG with a Lys164Gln mutation, gives rise to haemolytic disease of the newborn
    Tanaka M, Abe T, Minamitani T, Akiba H, Horikawa T, Tobita R, Isa K, Ogasawara K, Takahashi H, Tateyama H, Tone S, Tsumoto K, Yasui T, Kimura T, Fujimura Y, Hirayama F, Tani Y, Takihara Y
    Br. J. Haematol. 191, 920-926 (2020).
  • Dual-Sensitive Nanomicelles Enhancing Systemic Delivery of Therapeutically Active Antibodies Specifically into the Brain
    Xie J, Gonzalez-Carter D, Tockary TA, Nakamura N, Xue Y, Nakakido M, Akiba H, Dirisala A, Liu X, Toh K, Yang T, Wang Z, Fukushima S, Li J, Quader S, Tsumoto K, Yokota T, Anraku Y, Kataoka K
    ACS Nano, 14, 6729-6742 (2020).
  • 64Cu-labeled minibody D2101 visualizes CDH17-positive gastric cancer xenografts with short waiting time
    Fujiwara K, Akiba H, Tsuji AB, Sudo H, Sugyo A, Nagatsu K, Zhang MR, Iwanari H, Kusano-Arai O, Kudo S, Kikuchi C, Tsumoto K, Momose T, Hamakubo T, Higashi T
    Nucl. Med. Commun. 41, 688-695 (2020).
  • GPC1 specific CAR-T cells eradicate established solid tumor without adverse effects and synergize with anti-PD-1 Ab
    Kato D, Yaguchi T, Iwata T, Katoh Y, Morii K, Tsubota K, Takise Y, Tamiya M, Kamada H, Akiba H, Tsumoto K, Serada S, Naka T, Nishimura R, Nakagawa T, Kawakami Y
    eLife 9, e49392 (2020).
  • Computer-guided library generation applied to the optimization of single-domain antibodies
    Akiba H, Tamura H, Caaveiro JMM, Tsumoto K
    Prot. Eng. Des. Selec. 32, 423-431 (2020)
  • Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher
    Akiba H, Takayanagi K, Kusano-Arai O, Iwanari H, Hamakubo T, Tsumoto K
    Biotechnol. Rep. 25, e00418 (2020)


  • 111In-labeled anti-cadherin17 antibody D2101 has potential as a noninvasive imaging probe for diagnosing gastric cancer and lymph-node metastasis.
    Fujiwara K, Tsuji AB, Sudo H, Sugyo A, Akiba H, Iwanari H, Kusano-Arai O, Tsumoto K, Momose T, Hamakubo T, Higashi T
    Ann. Nucl. Med. 34, 13-23 (2019)
  • Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody.
    Akiba H, Tamura H, Kiyoshi M, Yanaka S, Sugase K, Caaveiro JMM, Tsumoto K
    Sci. Rep. 9, 15481 (2019)
  • Effect of allotypic variation of human IgG1 on the thermal stability of disulfide-linked knobs-into-holes mutants of the Fc for stable bispecific antibody design
    Akiba H, Satoh R, Nagata S, Tsumoto K
    Antib. Ther. 2, 65-69 (2019)
    Cover Image of the issue (


  • Structural behavior of keratin-associated protein 8.1 in human hair as revealed by a monoclonal antibody.
    Akiba H, Ikeuchi E, Ganbat J, Fujikawa H, Arai-Kusano O, Iwanari H, Nakakido M, Hamakubo T, Shimomura Y, Tsumoto K
    J. Struct. Biol. 204, 207-214 (2018)
  • Intramolecular H-bonds govern the recognition of a flexible peptide by an antibody.
    Miyanabe K, Akiba H, Kuroda D, Nakakido M, Kusano-Arai O, Iwanari H, Hamakubo T, Caaveiro JMM, Tsumoto K
    J. Biochem. 164, 65-76 (2018)
  • Tyrosine Sulfation Restricts the Conformational Ensemble of a Flexible Peptide, Strengthening the Binding Affinity for an Antibody.
    Miyanabe K, Yamashita T, Abe Y, Akiba H, Takamatsu Y, Nakakido M, Hamakubo T, Ueda T, Caaveiro JMM, Tsumoto K
    Biochemistry 57, 4177-4185 (2018)
  • Roles of the disulfide bond between the variable and the constant domains of rabbit immunoglobulin kappa chains in thermal stability and affinity.
    Kawade R, Akiba H, Entzminger K, Maruyama T, Okumura CJ, Tsumoto K
    Prot. Eng. Des. Selec. 31, 243-247 (2018)
  • Synergistic Cytotoxic Effect on Gastric Cancer Cells of an Immunotoxin Cocktail in Which Antibodies Recognize Different Epitopes on CDH17.
    Kusano-Arai O, Iwanari H, Kudo S, Kikuchi C, Yui A, Akiba H, Matsusaka K, Kaneda A, Fukayama M, Tsumoto K, Hamakubo T
    Monoclon. Antib. Immunodiagn. Immunother. 37, 1-11 (2018)

Books and Reviews

  • Development and activities, including immunocomplex formation, of biparatopic antibodies and alternative scaffold proteins
    Akiba H, Tsumoto K
    Translat. Regulat. Sci. 2, 1-6 (2020) (Review)

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