論文

2019年

• ¹¹¹In-labeled anti-cadherin17 antibody D2101 has potential as a noninvasive imaging probe for diagnosing gastric cancer and lymph-node metastasis.
  Fujiwara K, Tsuji AB, Sudo H, Sugyo A, Akiba H, Iwanari H, Kusano-Arai O, Tsumoto K, Momose T, Hamakubo T, Higashi T
  Ann. Nucl. Med. 34, 13-23 (2019)
  https://doi.org/10.1007/s12149-019-01408-y
• Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody.
  Akiba H, Tamura H, Kiyoshi M, Yanaka S, Sugase K, Caaveiro JMM, Tsumoto K
  Sci. Rep. 9, 15481 (2019)
  https://doi.org/10.1038/s41598-019-50722-y
• Effect of allotypic variation of human IgG1 on the thermal stability of disulfide-linked knobs-into-holes mutants of the Fc for stable bispecific antibody design
  Akiba H, Satoh R, Nagata S, Tsumoto K
  Antib. Ther. 2, 65-69 (2019)
  https://doi.org/10.1093/abt/tbz008
  Cover Image of the issue (https://academic.oup.com/abt/issue/2/3)

2018年

• Structural behavior of keratin-associated protein 8.1 in human hair as revealed by a monoclonal antibody.
  Akiba H, Ikeuchi E, Ganbat J, Fujikawa H, Arai-Kusano O, Iwanari H, Nakakido M, Hamakubo T, Shimomura Y, Tsumoto K
  J. Struct. Biol. 204, 207-214 (2018)
  https://doi.org/10.1016/j.jsb.2018.08.011
• Intramolecular H-bonds govern the recognition of a flexible peptide by an antibody.
  Miyanabe K, Akiba H, Kuroda D, Nakakido M, Kusano-Arai O, Iwanari H, Hamakubo T, Caaveiro JMM, Tsumoto K
  J. Biochem. 164, 65-76 (2018)
  https://doi.org/10.1093/jb/mvy032
  2019年日本生化学会JB論文賞 受賞
• Tyrosine Sulfation Restricts the Conformational Ensemble of a Flexible Peptide, Strengthening the Binding Affinity for an Antibody.
  Miyanabe K, Yamashita T, Abe Y, Akiba H, Takamatsu Y, Nakakido M, Hamakubo T, Ueda T, Caaveiro JMM, Tsumoto K
  Biochemistry 57, 4177-4185 (2018)
  https://doi.org/10.1021/acs.biochem.8b00592
• Roles of the disulfide bond between the variable and the constant domains of rabbit immunoglobulin kappa chains in thermal stability and affinity.
  Kawade R, Akiba H, Entzminger K, Maruyama T, Okumura CJ, Tsumoto K
  Prot. Eng. Des. Selec. 31, 243-247 (2018)
  https://doi.org/10.1093/protein/gzy008
• Synergistic Cytotoxic Effect on Gastric Cancer Cells of an Immunotoxin Cocktail in Which Antibodies Recognize Different Epitopes on CDH17.
  Kusano-Arai O, Iwanari H, Kudo S, Kikuchi C, Yui A, Akiba H, Matsusaka K, Kaneda A, Fukayama M, Tsumoto K, Hamakubo T
  Monoclon. Antib. Immunodiagn. Immunother. 37, 1-11 (2018)
  https://doi.org/10.1089/mab.2017.0043

2017年以前

• Thermodynamic analyses of amino acid residues at the interface of an antibody B2212A and its antigen roundabout homolog 1.
  Yui A, Akiba H, Kudo S, Nakakido M, Nagatoishi S, Tsumoto K
  J. Biochem. 162, 255-258 (2017)
• Use of SpyTag/SpyCatcher to construct bispecific antibodies that target two epitopes of a single antigen.
  Yumura K, Akiba H, Nagatoishi S, Kusano-Arai O, Iwanari H, Hamakubo T, Tsumoto K
  J. Biochem. 162, 203-210 (2017)
• Improved Brain Expression of Anti-Amyloid β scFv by Complexation of mRNA Including a Secretion Sequence with PEG-based Block Catiomer.
  Perche F, Uchida S, Akiba H, Lin CY, Ikegami M, Dirisala A, Nakashima T, Itaka K, Tsumoto K, Kataoka K
  Curr. Alzheimer Res. 14, 295-302 (2017)
• Click conjugation of a binuclear terbium(III) complex for real-time detection of tyrosine phosphorylation.
  Akiba H, Sumaoka J, Tsumoto K, Komiyama M
  Anal. Chem. 87, 3834-3840 (2015)
• Conjugation-free, visual, and quantitative evaluation of inhibitors on protein tyrosine kinases and phosphatases with a luminescent Tb(III) complex.
  Akiba H, Sumaoka J, Hamakubo T, Komiyama M
  Anal. Bioanal. Chem. 406, 2957-2964 (2014)
• Binuclear terbium(III) complex as a probe for tyrosine phosphorylation.
  Akiba H, Sumaoka J, Komiyama M
  Chem. Eur. J. 16, 5018-5025 (2010)
• Selective detection of phosphotyrosine in the presence of various phosphate-containing biomolecules with the aid of a terbium(III) complex.
  Akiba H, Sumaoka J, Komiyama M
  Chembiochem 10, 1773-1776 (2009)

総説・著書・邦文誌等

• 抗体の小分子化とドラッグデリバリーへの展開
  秋葉宏樹, 津本浩平
  月刊バイオインダストリー 33(2), 47-53 (2016)
• Selective Sensing of Tyrosine Phosphorylation in Peptides Using Terbium(III) Complexes.
  Sumaoka J, Akiba H, Komiyama M
  Int. J. Anal. Chem. 2016, 3216523 (2016) (Review)
• Thermodynamics of antibody-antigen interaction revealed by mutation analysis of antibody variable regions.
  Akiba H, Tsumoto K
  J. Biochem. 158, 1-13 (2015) (Review)

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